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Information Journal Paper

Title

Cytotoxic activity of caffeic acid and gallic acid against MCF-7 human breast cancer cells: An in silico and in vitro study

Pages

  574-586

Abstract

 Objective: Phenolic compounds have been considered inhibitors of various cancers. Material and Methods: In this study, Caffeic acid and Gallic acid were appraised for their possible effects on apoptotic genes expression in a breast cancer cell line in vitro. We also evaluated ligand interaction and ligand binding with estrogen receptor alpha by molecular docking. To determine half maximal inhibitory concentration, MCF-7 cells were treated with different concentrations of Caffeic acid and Gallic acid by 3-(4, 5dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Furthermore, morphological changes in cells and alterations in P53, Mcl-1 and P21 gene expression were studied by real-time RT-PCR. Also, protein network and different interactions between the desired genes were analyzed using GeneMANIA database. Results: Evaluation of cell survival by MTT assay revealed that the half-maximal inhibitory concentration values for Caffeic acid and Gallic acid against MCF-7 cells, were 159 and 18 µ g/ml, respectively. These compounds were found to affect P53, Mcl-1 and P21 gene expression; this alteration in gene expression probably occurred along with the activation of intrinsic apoptotic signaling pathway. Conclusion: Via apoptosis induction, Caffeic acid and Gallic acid have induce toxic effects and morphological changes in breast cancer cells, suggesting their possible future application as antitumor agents.

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    APA: Copy

    REZAEI SERESHT, HASAN, CHESHOMI, HAMID, Falanji, Farahnaz, MOVAHEDI MOTLAGH, FATEMEH, HASHEMIAN, MARYAM, & Mireskandari, Erfan. (2019). Cytotoxic activity of caffeic acid and gallic acid against MCF-7 human breast cancer cells: An in silico and in vitro study. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), 9(6), 574-586. SID. https://sid.ir/paper/326545/en

    Vancouver: Copy

    REZAEI SERESHT HASAN, CHESHOMI HAMID, Falanji Farahnaz, MOVAHEDI MOTLAGH FATEMEH, HASHEMIAN MARYAM, Mireskandari Erfan. Cytotoxic activity of caffeic acid and gallic acid against MCF-7 human breast cancer cells: An in silico and in vitro study. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)[Internet]. 2019;9(6):574-586. Available from: https://sid.ir/paper/326545/en

    IEEE: Copy

    HASAN REZAEI SERESHT, HAMID CHESHOMI, Farahnaz Falanji, FATEMEH MOVAHEDI MOTLAGH, MARYAM HASHEMIAN, and Erfan Mireskandari, “Cytotoxic activity of caffeic acid and gallic acid against MCF-7 human breast cancer cells: An in silico and in vitro study,” AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), vol. 9, no. 6, pp. 574–586, 2019, [Online]. Available: https://sid.ir/paper/326545/en

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