PROTECTIVE ROLE OF LITHIUM CHLORIDE AGAINST INDUCED APOPTOSIS IN MESENCHYMAL STEM CELLS DERIVED FROM RAT MARROW AT THE CULTURE

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BAGHBAN ESLAMINEZHAD M.R.; TALKHABI M.; ZEYNALI B.; EFTEKHARI YAZDI P.

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Journal: KOWSAR MEDICAL JOURNAL Year:2009 | Volume:13 | Issue:4 Page(s): 259-266

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Title

PROTECTIVE ROLE OF LITHIUM CHLORIDE AGAINST INDUCED APOPTOSIS IN MESENCHYMAL STEM CELLS DERIVED FROM RAT MARROW AT THE CULTURE

Author(s)

BAGHBAN ESLAMINEZHAD M.R. | TALKHABI M. | ZEYNALI B. | EFTEKHARI YAZDI P. | Issue Writer Certificate

Keywords

RAT MESENCHYMAL STEM CELLSQ3

APOPTOSISQ3

SERUM DEPRIVATIONQ3

TNF-αQ3

Abstract

AIMS: The objective of this study is to examine the protective effects of lithium chloride against induced APOPTOSIS in RAT MESENCHYMAL STEM CELLS (MSCs) culture.METHODS: In this experimental study, rat bone marrow cells were plated in 2, 5 and 10 mM of lithium chloride and expanded for three successive subcultures. At the end of cultivation period, the number of viable cells in treatment groups and control (without lithium chloride) was determined and compared using propidium Iodide (PI) staining. Furthermore, at passaged-3 cultures, the cells were induced by SERUM DEPRIVATION and TNF- addition and concomitantly treated by the mentioned concentration of lithium chloride. At 24, 48 and 72 hours after APOPTOSIS induction, the cultures were stained with Acridine Orange and Ethidium Bromide.RESULTS: Flowcytometric distribution of dead and living cells after PI staining indicated that treatment of cells with varying concentration of Lithium cause the increase in number of viable cells compared to that of control. In this term, the highest number of viable cells belongs to culture with 6 mM Lithium chloride (p<0.05). Percentages of apoptotic cells for control, 2, 5 and 10 mM lithium were respectively, 19.34, 13.55, 5.75 and 10.31 after 24h that increased by time advancement (48 and 72 hours), cause more differences. In this regards, the least percentages belongs to culture with 5 mM of lithium (p<0.001).CONCLUSION: Lithium chloride, especially at 5 mM concentration, can decrease the amount of apoptotic cells at MSCs cultures that are induced to undergo APOPTOSIS.

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APA: Copy

BAGHBAN ESLAMINEZHAD, M.R., TALKHABI, M., ZEYNALI, B., & EFTEKHARI YAZDI, P.. (2009). PROTECTIVE ROLE OF LITHIUM CHLORIDE AGAINST INDUCED APOPTOSIS IN MESENCHYMAL STEM CELLS DERIVED FROM RAT MARROW AT THE CULTURE. KOWSAR MEDICAL JOURNAL, 13(4), 259-266. SID. https://sid.ir/paper/32881/en

Vancouver: Copy

BAGHBAN ESLAMINEZHAD M.R., TALKHABI M., ZEYNALI B., EFTEKHARI YAZDI P.. PROTECTIVE ROLE OF LITHIUM CHLORIDE AGAINST INDUCED APOPTOSIS IN MESENCHYMAL STEM CELLS DERIVED FROM RAT MARROW AT THE CULTURE. KOWSAR MEDICAL JOURNAL[Internet]. 2009;13(4):259-266. Available from: https://sid.ir/paper/32881/en

IEEE: Copy

M.R. BAGHBAN ESLAMINEZHAD, M. TALKHABI, B. ZEYNALI, and P. EFTEKHARI YAZDI, “PROTECTIVE ROLE OF LITHIUM CHLORIDE AGAINST INDUCED APOPTOSIS IN MESENCHYMAL STEM CELLS DERIVED FROM RAT MARROW AT THE CULTURE,” KOWSAR MEDICAL JOURNAL, vol. 13, no. 4, pp. 259–266, 2009, [Online]. Available: https://sid.ir/paper/32881/en

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