مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

214
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

346
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

PROTECTIVE EFFECT OF DIOSGENIN AGAINST H2O2-INDUCED OXIDATIVE STRESS ON H9C2 CELLS

Pages

  58-64

Abstract

 DIOSGENIN is an important compound in pharmaceutical industry. It has various effects such as hypocholesterolemic action or antioxidant activity in HIV infected patients. Biological oxidation pathways are involved in causing or aggravating heart disease. This study investigated the potential protective effect of DIOSGENIN on CELL VIABILITY and antioxidant defenses of cultured H9C2 CELLS submitted to OXIDATIVE STRESS induced by H2O2. Viability of cells exposed to H2O2 was detected by MTT assay. The generation of ROS and hydrogen peroxide release after H2O2 were detected using the fluorescent probe H2DCF-DA. The lipid peroxidation product i.e. MDA formation was estimated by assessing the levels of thio-barbituric acid reactive substances (TBARS) using spectrophotometry. SOD activity was assayed with NWLSS (TM) Superoxide Dismutase (SOD) activity assay kit. Pretreatment of cells with 3-25 mM of DIOSGENIN for 24 h before applying H2O2 completely prevented cell damage and significantly enhanced viability of H9C2 CELLS. Increased ROS induced by H2O2 was dose dependently prevented when cells were pretreated for 24 h with DIOSGENIN. The level of the lipid peroxidation was significantly higher in H9C2 CELLS exposed to H2O2 as compared to the control and cells pretreated with DIOSGENIN. SOD activity in cells treated with DIOSGENIN significantly decreased compared with cells exposed to H2O2. These results show that treatment of H9C2 CELLS with DIOSGENIN (3-25 mM) confers a significant protection against OXIDATIVE STRESS.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    JAMSHIDI, SAMANEH, LAHOUTI, MEHRDAD, BOROUSHAKI, MOHAMMAD TAHER, GANJEALI, A., GHORBANI, AHMAD, & BIHAMTA TOOSI, MEHDI. (2016). PROTECTIVE EFFECT OF DIOSGENIN AGAINST H2O2-INDUCED OXIDATIVE STRESS ON H9C2 CELLS. JOURNAL OF CELL AND MOLECULAR RESEARCH, 8(2), 58-64. SID. https://sid.ir/paper/329278/en

    Vancouver: Copy

    JAMSHIDI SAMANEH, LAHOUTI MEHRDAD, BOROUSHAKI MOHAMMAD TAHER, GANJEALI A., GHORBANI AHMAD, BIHAMTA TOOSI MEHDI. PROTECTIVE EFFECT OF DIOSGENIN AGAINST H2O2-INDUCED OXIDATIVE STRESS ON H9C2 CELLS. JOURNAL OF CELL AND MOLECULAR RESEARCH[Internet]. 2016;8(2):58-64. Available from: https://sid.ir/paper/329278/en

    IEEE: Copy

    SAMANEH JAMSHIDI, MEHRDAD LAHOUTI, MOHAMMAD TAHER BOROUSHAKI, A. GANJEALI, AHMAD GHORBANI, and MEHDI BIHAMTA TOOSI, “PROTECTIVE EFFECT OF DIOSGENIN AGAINST H2O2-INDUCED OXIDATIVE STRESS ON H9C2 CELLS,” JOURNAL OF CELL AND MOLECULAR RESEARCH, vol. 8, no. 2, pp. 58–64, 2016, [Online]. Available: https://sid.ir/paper/329278/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button