Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Aftabi Younes; Zarredar Habib; Sheikhi Mohammadreza; KHOSHKAM ZAHRA; HOSSEINZADEH COLAGAR ABASALT

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CELL AND MOLECULAR RESEARCH Year:2019 | Volume:10 | Issue:2 Page(s): 67-75

Download Full-Text

View:

179

Download:

155

Cites:

Information Journal Paper

Title

Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I

Author(s)

Keywords

Aryl hydrocarbon receptor

Colon cancer

Cucurbitacin

HT-29

SW-480

Abstract

Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor and its induction may result in suppressing of cell proliferation in colorectal cancer (CRC). Cucurbitacin D (CucD), E (CucE) and I (CucI) are plant derived metabolites that inhibit cancer cells. This study aimed to evaluate the possible potency of the Cucurbitacins for activation of AHR expression in CRC cell lines SW-480 and HT-29. The MTT assay was used to find the IC50 value of the metabolites in the cell lines. Afterwards, the cells were incubated with the IC50 concentrations of the Cucurbitacins and AHR-mRNA expression assessed using RT-PCR. The IC50 values of CucD, CucE, and CucI were 4. 5, 6. 8, and 3. 8 μ M in HT-29 cell line and 35, 19, 17. 5 μ M in SW-480 cells, respectively. The SW-480 cells were more resistant against Cucurbitacins in comparison with HT-29 cells and all three Cucurbitacins led to more AHRmRNA expression in HT-29 cells. CucE had the lowest effect on AHR-mRNA expression in the cell lines and CucI was a common metabolite for both HT-29 and SW-480 cells, which showed the lowest IC50 value (the highest toxicity) and the highest effect on AHR-mRNA expression. CucI may have a potential AHR-induction role and it could be applicable as an AHR-expression inducer in CRC studies.

Cites

No record.

References

Cite

APA: Copy

Aftabi, Younes, Zarredar, Habib, Sheikhi, Mohammadreza, KHOSHKAM, ZAHRA, & HOSSEINZADEH COLAGAR, ABASALT. (2019). Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I. JOURNAL OF CELL AND MOLECULAR RESEARCH, 10(2), 67-75. SID. https://sid.ir/paper/329313/en

Vancouver: Copy

Aftabi Younes, Zarredar Habib, Sheikhi Mohammadreza, KHOSHKAM ZAHRA, HOSSEINZADEH COLAGAR ABASALT. Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I. JOURNAL OF CELL AND MOLECULAR RESEARCH[Internet]. 2019;10(2):67-75. Available from: https://sid.ir/paper/329313/en

IEEE: Copy

Younes Aftabi, Habib Zarredar, Mohammadreza Sheikhi, ZAHRA KHOSHKAM, and ABASALT HOSSEINZADEH COLAGAR, “Induction of AHR Gene Expression in Colorectal Cancer Cell Lines by Cucurbitacin D, E, and I,” JOURNAL OF CELL AND MOLECULAR RESEARCH, vol. 10, no. 2, pp. 67–75, 2019, [Online]. Available: https://sid.ir/paper/329313/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops