PURIFICATION OF LIPOPOLYSACCHARIDES FROM SALMONELLA TYPHIMURIUM PTCC 1735

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HOSSEINI JAZANI N.; BEHZADIANNEZHAD GH.; ABDI AALI A.; TABARAEI BAHMAN; MOHAMMAD HASAN ZOHEYR

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Journal: KOWSAR MEDICAL JOURNAL Year:2003 | Volume:7 | Issue:part 4 Page(s): 261-268

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Title

PURIFICATION OF LIPOPOLYSACCHARIDES FROM SALMONELLA TYPHIMURIUM PTCC 1735

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Keywords

LIPOPOLYSACCHARIDEQ3

SALMONELLA TYPHIMURIUMQ4

PURIFICATIONQ4

Abstract

LIPOPOLYSACCHARIDE (LPS) is one of the most important components of Gram negative bacteria cell walls which form a hydrophobic unit (Lipid A) and a hydrophilic unit (polyssaccharide ). Extraction and PURIFICATION of LPS is an essential step in determination of biochemical, pathophysiological and immunological properties. In the present report the LPS of SALMONELLA TYPHIMURIUM PTCC 1735 was extracted and purified. The phenol-water solution method was used to extract the bacterial LPS. Proteins and nucleic acids were removed by means of pronase and nucleases and gel filteration. Crude LPS yield was about 1.8% of initial bacterial dry weight. after gel filteration the content of protein and nucleic acids in LPS preparations were calculated to be 0.2% and 0.5% respectively. Profiles of pure LPS by SDS-PAGE were demonstrated.

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APA: Copy

HOSSEINI JAZANI, N., BEHZADIANNEZHAD, GH., ABDI AALI, A., TABARAEI, BAHMAN, & MOHAMMAD HASAN, ZOHEYR. (2003). PURIFICATION OF LIPOPOLYSACCHARIDES FROM SALMONELLA TYPHIMURIUM PTCC 1735. KOWSAR MEDICAL JOURNAL, 7(part 4), 261-268. SID. https://sid.ir/paper/32949/en

Vancouver: Copy

HOSSEINI JAZANI N., BEHZADIANNEZHAD GH., ABDI AALI A., TABARAEI BAHMAN, MOHAMMAD HASAN ZOHEYR. PURIFICATION OF LIPOPOLYSACCHARIDES FROM SALMONELLA TYPHIMURIUM PTCC 1735. KOWSAR MEDICAL JOURNAL[Internet]. 2003;7(part 4):261-268. Available from: https://sid.ir/paper/32949/en

IEEE: Copy

N. HOSSEINI JAZANI, GH. BEHZADIANNEZHAD, A. ABDI AALI, BAHMAN TABARAEI, and ZOHEYR MOHAMMAD HASAN, “PURIFICATION OF LIPOPOLYSACCHARIDES FROM SALMONELLA TYPHIMURIUM PTCC 1735,” KOWSAR MEDICAL JOURNAL, vol. 7, no. part 4, pp. 261–268, 2003, [Online]. Available: https://sid.ir/paper/32949/en

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