INVESTIGATION OF ACETAMINOPHEN INDUCTION EFFECTS ON GSH CONCENTRATION BY N-ACETYLCYSTEINE AND 2-OXOTHRAZOLIDIN-4- CARBOXYLATE (OTC) AND ITS PROTECTIVE EFFECT ON SULFURE MUSTARD INJURES IN HF2FF CELLS

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ZAREEI MAHMOUDABADI A.B.; SABERI M.; PIR ZAD J.; GOLMANESH L.

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Journal: KOWSAR MEDICAL JOURNAL Year:2005 | Volume:10 | Issue:3 Page(s): 175-182

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Title

INVESTIGATION OF ACETAMINOPHEN INDUCTION EFFECTS ON GSH CONCENTRATION BY N-ACETYLCYSTEINE AND 2-OXOTHRAZOLIDIN-4- CARBOXYLATE (OTC) AND ITS PROTECTIVE EFFECT ON SULFURE MUSTARD INJURES IN HF2FF CELLS

Author(s)

ZAREEI MAHMOUDABADI A.B. | SABERI M. | PIR ZAD J. | GOLMANESH L. | Issue Writer Certificate

Keywords

GSHQ4

GSSGRQ4

ΓGCSQ4

OTCQ4

NACQ4

Abstract

Objective: The NAC and OTC effects in the presence of acetaminophen as an oxidative stress on GSH concentration in cultured HF2FF cells were studied. The protective effect of GSH on sulfur mustard injuries in cultured cells was also investigated. Materials and Methods: In this research the effects of N-acetlcysteine (NAC) and OTC with presence of an inducer such as acetaminophen on intracellular Glutathione (GSH) and γ -GCS and GSSGR activities in induced hemogenate were investigated. After induction of GSH concentration in cells, induced, normal and control cells were expressed with 180 μM HD for 1h and after washing, fresh media were added and incubated 24h, the cell viability was investigated by the GV method. Results: The addition of 0.1 mM NAC to HF2FF cells incubated with 25mM acetaminophen increased the GSH, γ-GCS and GSSGR activities respectively from 0.331±0.12 M/mg protein to 0.784±0.11 M/mg protein, 11.7±3.25 nmol NADH/min/mg protein to 39.8±4 nmol NADH/min/mg protein and 0.962±0.7 nmol NADPH/min/mg protein. Incubation of both NAC and OTC (0.1mM and 1mM) augmented the GSH level to 0.962±0.7M/mg protein and γ -GCS activity to 112.28 ± 11.85 nmol NADH/min/mg protein, but, GSSGR activity was not significantly increased. The viability of induced cell compared to normal in the presence of 180 μM of HD was significant (P<0.001), (47% to 73%), which was 1.55 times greater in viability. Discussion: These observations indicate that the NAC not only act as a precursor for GSH but also increased GSSGR activity which converted GSSG to GSH. On the other hand, OTC increased the rate-limiting enzyme (γ -GCS) in GSH biosynthesis pathway which increased the GSH levels without inhibition of γ -GCS. It is probably that OTC induces γ -GCS expression.

Cites

THE EFFECTS OF HYDROALCOHOLIC EXTRACT OF PORTULACA OLERACEA ON THE SERUM CONCENTREATION OF HEPATIC ENZYMES IN RATS

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APA: Copy

ZAREEI MAHMOUDABADI, A.B., SABERI, M., PIR ZAD, J., & GOLMANESH, L.. (2005). INVESTIGATION OF ACETAMINOPHEN INDUCTION EFFECTS ON GSH CONCENTRATION BY N-ACETYLCYSTEINE AND 2-OXOTHRAZOLIDIN-4- CARBOXYLATE (OTC) AND ITS PROTECTIVE EFFECT ON SULFURE MUSTARD INJURES IN HF2FF CELLS. KOWSAR MEDICAL JOURNAL, 10(3), 175-182. SID. https://sid.ir/paper/32976/en

Vancouver: Copy

ZAREEI MAHMOUDABADI A.B., SABERI M., PIR ZAD J., GOLMANESH L.. INVESTIGATION OF ACETAMINOPHEN INDUCTION EFFECTS ON GSH CONCENTRATION BY N-ACETYLCYSTEINE AND 2-OXOTHRAZOLIDIN-4- CARBOXYLATE (OTC) AND ITS PROTECTIVE EFFECT ON SULFURE MUSTARD INJURES IN HF2FF CELLS. KOWSAR MEDICAL JOURNAL[Internet]. 2005;10(3):175-182. Available from: https://sid.ir/paper/32976/en

IEEE: Copy

A.B. ZAREEI MAHMOUDABADI, M. SABERI, J. PIR ZAD, and L. GOLMANESH, “INVESTIGATION OF ACETAMINOPHEN INDUCTION EFFECTS ON GSH CONCENTRATION BY N-ACETYLCYSTEINE AND 2-OXOTHRAZOLIDIN-4- CARBOXYLATE (OTC) AND ITS PROTECTIVE EFFECT ON SULFURE MUSTARD INJURES IN HF2FF CELLS,” KOWSAR MEDICAL JOURNAL, vol. 10, no. 3, pp. 175–182, 2005, [Online]. Available: https://sid.ir/paper/32976/en

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