IDENTIFICATION OF IMPORTANT PATHOGENIC YEAST CANDIDA SPECIES IN ACUTE CANDIDIASIS USING PCR

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ESKANDARI AZADEH; MESBAH NAMIN S.A.R.; YADGARI M.H.

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Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2008 | Volume:13 | Issue:2 Page(s): 115-124

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Title

IDENTIFICATION OF IMPORTANT PATHOGENIC YEAST CANDIDA SPECIES IN ACUTE CANDIDIASIS USING PCR

Author(s)

ESKANDARI AZADEH | MESBAH NAMIN S.A.R. | YADGARI M.H. | Issue Writer Certificate

Keywords

CANDIDA ALBICANSQ3

CANDIDIASISQ3

CANDIDA SPECIESQ3

RAPID DIAGNOSISQ3

PCRQ3

Abstract

Objective: Rapid identification of important pathogenic candida fungi species by one round of PCR and evaluation of frequencies of them in acute CANDIDIASIS. Materials and methods: Four isolated and standard CANDIDA SPECIES were prepared first and after phenotyping, DNA extraction was performed on these samples. PCR was also optimized using a specific pair of primers on the CHS1 gene to obtain different fragments of the CANDIDA SPECIES. Sixty samples from patients with acute CANDIDIASIS were obtained. All of procedures were performed for clinical specimens. Results: The results of genotyping of 60 samples were as follows: 48 cases for C. Albicans, 4 cases for C. tropicalis, 2 cases for were C. Parapsilosis and 1 case for C. Glabrata. Only 5 out of 60 samples didn’t amplify in PCR and the phenotyping study showed that 3 samples of them were C. Krusei and 2 remaining samples might be another species of candida such as C. Dubliniensisc.Conclusion: The most fungal infection has emerged from CANDIDA SPECIES. Rapid detection of these species is important in the clinical laboratories. In this study 83.3% of samples with acute CANDIDIASIS were well-recognized with one round PCR which the CANDIDA ALBICANS has consisted 80% of the samples. Before treatment with drugs, screening the CANDIDIASIS samples with the rapid mentioned procedure is recommended by this study to do in any clinical centre. For identification of the rare pathogenic CANDIDIASIS, the conventional phenotypic methods could be used. The more comprehensive study with more samples could be able to demonstrate importance of this procedure.

Cites

CHEMICAL COMPOSITION AND ANTI-CANDIDA ACTIVITY OF TRACHYSPERMUM AMMI ESSENTIAL OIL ON AZOLES RESISTANT CANDIDA ALBICANS ISOLATES FROM ORAL CAVITY OF HIV+ PATIENTS

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APA: Copy

ESKANDARI, AZADEH, MESBAH NAMIN, S.A.R., & YADGARI, M.H.. (2008). IDENTIFICATION OF IMPORTANT PATHOGENIC YEAST CANDIDA SPECIES IN ACUTE CANDIDIASIS USING PCR. KOWSAR MEDICAL JOURNAL, 13(2), 115-124. SID. https://sid.ir/paper/33008/en

Vancouver: Copy

ESKANDARI AZADEH, MESBAH NAMIN S.A.R., YADGARI M.H.. IDENTIFICATION OF IMPORTANT PATHOGENIC YEAST CANDIDA SPECIES IN ACUTE CANDIDIASIS USING PCR. KOWSAR MEDICAL JOURNAL[Internet]. 2008;13(2):115-124. Available from: https://sid.ir/paper/33008/en

IEEE: Copy

AZADEH ESKANDARI, S.A.R. MESBAH NAMIN, and M.H. YADGARI, “IDENTIFICATION OF IMPORTANT PATHOGENIC YEAST CANDIDA SPECIES IN ACUTE CANDIDIASIS USING PCR,” KOWSAR MEDICAL JOURNAL, vol. 13, no. 2, pp. 115–124, 2008, [Online]. Available: https://sid.ir/paper/33008/en

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