DETECTION AND IDENTIFICATION OF COMMON BACTERIAL PATHOGENS BY PCR-RFLP

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MELLAT M.; MOUSAVI M.; AMANI J.; NAZARIYAN SH.

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Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:11 | Issue:2 Page(s): 125-133

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Information Journal Paper

Title

DETECTION AND IDENTIFICATION OF COMMON BACTERIAL PATHOGENS BY PCR-RFLP

Author(s)

MELLAT M. | MOUSAVI M. | AMANI J. | NAZARIYAN SH. | Issue Writer Certificate

Keywords

RAPID DETECTIONQ3

UNIVERSAL PCRQ3

16SRRNAQ3

PREVALENT PATHOGENIC BACTERIAQ3

Abstract

Objective: Using 16s rRNA as a target, in this research we tried to detect the PREVALENT PATHOGENIC BACTERIA from clinical sample. The gene coding for 16SRRNA of most of the pathogenic bacteria have been sequenced. These genes from different bacteria have some conserve sequences and by designing a pair of universal primers one can amplify this gene from different bacteria. Since the amplified products show different pattern when digested with restriction enzyme, so this technique can be used for detection of different pathogenic bacteria. We tried the same technique for detection of some standard and clinical samples and compared its sensitivity with that of culture methods. Material and Methods: Bacterial strains used in this study were collected either from Bo Ali Hospital (Iranian reference center) or Department of Medical Biotechnology-Tarbiat Modares University. Related clinical samples were obtained from different hospitals in Tehran. Taq DNA polymerase and restriction endonucleases were from Cinagene Company. With the help of a pair of universal primers, the gene coding for 16SRRNA from nine different bacteria were amplified. The bacteria were then identified from digestion pattern obtained by digestion with restriction endonuclease. Result: The results show that the nine pathogenic bacteria used in this investigation could be detected and identified by this technique. Using 50 clinical samples, the sensitivity of this method was %92 compared with culture method. Conclusions: Simultaneous testing for multiple pathogens is advantageous when the clinical symptoms for identifying the causative agent are not effective. The techniques used in this research, not only have the above advantage but also shows high sensitivity and is fast enough as compared with traditional methods. PCR-RFLP seems to be a reliable technique along with other methods for the detection of pathogenic bacteria.

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APA: Copy

MELLAT, M., MOUSAVI, M., AMANI, J., & NAZARIYAN, SH.. (2006). DETECTION AND IDENTIFICATION OF COMMON BACTERIAL PATHOGENS BY PCR-RFLP. KOWSAR MEDICAL JOURNAL, 11(2), 125-133. SID. https://sid.ir/paper/33027/en

Vancouver: Copy

MELLAT M., MOUSAVI M., AMANI J., NAZARIYAN SH.. DETECTION AND IDENTIFICATION OF COMMON BACTERIAL PATHOGENS BY PCR-RFLP. KOWSAR MEDICAL JOURNAL[Internet]. 2006;11(2):125-133. Available from: https://sid.ir/paper/33027/en

IEEE: Copy

M. MELLAT, M. MOUSAVI, J. AMANI, and SH. NAZARIYAN, “DETECTION AND IDENTIFICATION OF COMMON BACTERIAL PATHOGENS BY PCR-RFLP,” KOWSAR MEDICAL JOURNAL, vol. 11, no. 2, pp. 125–133, 2006, [Online]. Available: https://sid.ir/paper/33027/en

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