CLONING AND EXPRESSION OF IPAC GENE FROM SHIGELLA DYSENTERIAE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MALLAEI F.; SAADATI MOJTABA; HONARI HOSSEIN; NAZARIYAN SH.; EGHTEDAR DOUST M.; HEIAT M.; HESARAKI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2011 | Volume:16 | Issue:1 Page(s): 1-6

Download Full-Text

Persian Verion

View:

1,462

Download:

Cites:

Information Journal Paper

Title

CLONING AND EXPRESSION OF IPAC GENE FROM SHIGELLA DYSENTERIAE

Author(s)

Keywords

CLONINGQ3

EXPRESSIONQ2

IPAC GENEQ2

SHIGELLA DYSENTERIAEQ3

RECOMBINANT VACCINEQ2

SHIGELLOSISQ2

Abstract

Aims: SHIGELLOSIS is caused by Shigella species. Considering the high frequency of morbidity and mortality reports and antibiotics resistance, designing and producing a vaccine against this disease is one of the goals of World Health Organization. Invasion Plasmid Antigen especially IpaC and IpaB are the major Shigella virulence agents and are also vaccine candidates. The aim of this study was to express IPAC GENE which is of the virulence factors in order to investigate its immunogenicity. Materials & Methods: In this study, IPAC GENE was obtained from NCBI gene bank and primers were designed. After genome extraction from SHIGELLA DYSENTERIAE, it was used as template for PCR amplification. The amplified IPAC GENE by PCR was cloned into pTZ57R and sub-cloned into the EXPRESSION vector pET-28a(+). E. coli BL21(DE3)plysS was transformed by recombinant vector pET-28a(+)/ipaC and EXPRESSION of the recombinant ipaC was investigated by IPTG induction and SDS-PAGE electrophoresis. Results: CLONING was confirmed by PCR, enzyme digestion and sequencing. In addition, the recombinant protein was expressed by IPTG induction. Protein EXPRESSION was confirmed by Ni–NTA column, Western- Blotting analysis and SDS-PAGE electrophoresis.Conclusion: CLONING, sub-CLONING and EXPRESSION of IPAC GENE are confirmed in the present study. Therefore, this recombinant protein can be used for production of a RECOMBINANT VACCINE against SHIGELLA DYSENTERIAE in future studies, after immunogenicity assay.

Cites

No record.

References

No record.

Cite

APA: Copy

MALLAEI, F., SAADATI, MOJTABA, HONARI, HOSSEIN, NAZARIYAN, SH., EGHTEDAR DOUST, M., HEIAT, M., & HESARAKI, M.. (2011). CLONING AND EXPRESSION OF IPAC GENE FROM SHIGELLA DYSENTERIAE. KOWSAR MEDICAL JOURNAL, 16(1), 1-6. SID. https://sid.ir/paper/33041/en

Vancouver: Copy

MALLAEI F., SAADATI MOJTABA, HONARI HOSSEIN, NAZARIYAN SH., EGHTEDAR DOUST M., HEIAT M., HESARAKI M.. CLONING AND EXPRESSION OF IPAC GENE FROM SHIGELLA DYSENTERIAE. KOWSAR MEDICAL JOURNAL[Internet]. 2011;16(1):1-6. Available from: https://sid.ir/paper/33041/en

IEEE: Copy

F. MALLAEI, MOJTABA SAADATI, HOSSEIN HONARI, SH. NAZARIYAN, M. EGHTEDAR DOUST, M. HEIAT, and M. HESARAKI, “CLONING AND EXPRESSION OF IPAC GENE FROM SHIGELLA DYSENTERIAE,” KOWSAR MEDICAL JOURNAL, vol. 16, no. 1, pp. 1–6, 2011, [Online]. Available: https://sid.ir/paper/33041/en

Related Journal Papers