مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

NUCLEOSTEMIN DEPLETION INDUCES POST-G1 ARREST APOPTOSIS IN CHRONIC MYELOGENOUS LEUKEMIA K562 CELLS

Pages

  55-60

Abstract

 Purpose: Despite significant improvements in treatment of CHRONIC MYELOGENOUS LEUKEMIA (CML), the emergence of leukemic stem cell (LSC) concept questioned efficacy of current therapeutical protocols. Remaining issue on CML includes finding and targeting of the key genes responsible for self-renewal and proliferation of LSCs. NUCLEOSTEMIN (NS) is a new protein localized in the nucleolus of most stem cells and tumor cells which regulates their self-renewal and CELL CYCLE progression. The aim of this study was to investigate effects of NS knocking down in K562 cell line as an in vitro model of CML.Methods: NS gene silencing was performed using a specific small interfering RNA (NS-siRNA). The gene expression level of NS was evaluated by RT-PCR. The viability and growth rate of K562 cells were determined by trypan blue exclusion test. CELL CYCLE distribution of the cells was analyzed by flow cytometry.Results: Our results showed that NS knocking down inhibited proliferation and viability of K562 cells in a time-dependent manner. CELL CYCLE studies revealed that NS depletion resulted in G1 CELL CYCLE arrest at short times of transfection (24 h) followed with APOPTOSIS at longer times (48 and 72 h), suggest that post-G1 arrest APOPTOSIS is occurred in K562 cells.Conclusion: Overall, these results point to essential role of NS in K562 cells, thus, this gene might be considered as a promising target for treatment of CML.

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    APA: Copy

    SEYED GOGANI, NEGIN, RAHMATI, MARVEH, ZARGHAMI, NOSRATOLLAH, ASVADI KERMANI, IRAJ, HOSEINPOUR FEYZI, MOHAMMAD ALI, & MOOSAVI, MOHAMMAD AMIN. (2014). NUCLEOSTEMIN DEPLETION INDUCES POST-G1 ARREST APOPTOSIS IN CHRONIC MYELOGENOUS LEUKEMIA K562 CELLS. ADVANCED PHARMACEUTICAL BULLETIN, 4(1), 55-60. SID. https://sid.ir/paper/331736/en

    Vancouver: Copy

    SEYED GOGANI NEGIN, RAHMATI MARVEH, ZARGHAMI NOSRATOLLAH, ASVADI KERMANI IRAJ, HOSEINPOUR FEYZI MOHAMMAD ALI, MOOSAVI MOHAMMAD AMIN. NUCLEOSTEMIN DEPLETION INDUCES POST-G1 ARREST APOPTOSIS IN CHRONIC MYELOGENOUS LEUKEMIA K562 CELLS. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2014;4(1):55-60. Available from: https://sid.ir/paper/331736/en

    IEEE: Copy

    NEGIN SEYED GOGANI, MARVEH RAHMATI, NOSRATOLLAH ZARGHAMI, IRAJ ASVADI KERMANI, MOHAMMAD ALI HOSEINPOUR FEYZI, and MOHAMMAD AMIN MOOSAVI, “NUCLEOSTEMIN DEPLETION INDUCES POST-G1 ARREST APOPTOSIS IN CHRONIC MYELOGENOUS LEUKEMIA K562 CELLS,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 4, no. 1, pp. 55–60, 2014, [Online]. Available: https://sid.ir/paper/331736/en

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