THERAPEUTIC EFFECTS OF MYELOID CELL LEUKEMIA-1 SIRNA ON HUMAN ACUTE MYELOID LEUKEMIA CELLS

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KARAMI HADI; BARADARAN BEHZAD; ESFAHANI ALI; SAKHINIA MASOUD; SAKHINIA EBRAHIM

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Journal: Advanced Pharmaceutical Bulletin Year:2014 | Volume:4 | Issue:3 Page(s): 243-248

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Title

THERAPEUTIC EFFECTS OF MYELOID CELL LEUKEMIA-1 SIRNA ON HUMAN ACUTE MYELOID LEUKEMIA CELLS

Author(s)

Keywords

MCL-1

SIRNA

LEUKEMIA

HL-60

APOPTOSIS

PROLIFERATION

Abstract

Purpose: Up-regulation of MCL-1, a known anti-apoptotic protein, is associated with the survival and progression of various malignancies including LEUKEMIA. The aim of this study was to explore the effect of MCL-1 small interference RNA (siRNA) on the PROLIFERATION and APOPTOSIS of HL-60 acute myeloid LEUKEMIA (AML) cells.Methods: SIRNA transfection was performed using LipofectamineTM 2000 reagent. Relative mRNA and protein expressions were quantified by quantitative real-time PCR and Western blotting, respectively. Trypan blue assay was performed to assess tumor cell PROLIFERATION after SIRNA transfection. The cytotoxic effect of MCL-1 SIRNA on leukemic cells was measured using MTT assay. APOPTOSIS was detected using ELISA cell death assay.Results: MCL-1 SIRNA clearly lowered both MCL-1 mRNA and protein levels in a time-dependent manner, leading to marked inhibition of cell survival and PROLIFERATION. Furthermore, MCL-1 down-regulation significantly enhanced the extent of HL-60 apoptotic cells.Conclusion: Our results suggest that the down-regulation of MCL-1 by SIRNA can effectively trigger APOPTOSIS and inhibit the PROLIFERATION of leukemic cells. Therefore, MCL-1 SIRNA may be a potent adjuvant in AML therapy.

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APA: Copy

KARAMI, HADI, BARADARAN, BEHZAD, ESFAHANI, ALI, SAKHINIA, MASOUD, & SAKHINIA, EBRAHIM. (2014). THERAPEUTIC EFFECTS OF MYELOID CELL LEUKEMIA-1 SIRNA ON HUMAN ACUTE MYELOID LEUKEMIA CELLS. ADVANCED PHARMACEUTICAL BULLETIN, 4(3), 243-248. SID. https://sid.ir/paper/331768/en

Vancouver: Copy

KARAMI HADI, BARADARAN BEHZAD, ESFAHANI ALI, SAKHINIA MASOUD, SAKHINIA EBRAHIM. THERAPEUTIC EFFECTS OF MYELOID CELL LEUKEMIA-1 SIRNA ON HUMAN ACUTE MYELOID LEUKEMIA CELLS. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2014;4(3):243-248. Available from: https://sid.ir/paper/331768/en

IEEE: Copy

HADI KARAMI, BEHZAD BARADARAN, ALI ESFAHANI, MASOUD SAKHINIA, and EBRAHIM SAKHINIA, “THERAPEUTIC EFFECTS OF MYELOID CELL LEUKEMIA-1 SIRNA ON HUMAN ACUTE MYELOID LEUKEMIA CELLS,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 4, no. 3, pp. 243–248, 2014, [Online]. Available: https://sid.ir/paper/331768/en

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