PURIFICATION AND CHARACTERIZATION OF BOVINE SERUM ALBUMIN USING CHROMATOGRAPHIC METHOD (SHORT COMMUNICATION)

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balkani Sanaz; Shamekhi Sara; Raoufinia Ramin; Parvan Reza; ABDOLALIZADEH JALAL

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Journal: Advanced Pharmaceutical Bulletin Year:2016 | Volume:6 | Issue:4 Page(s): 651-654

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Title

PURIFICATION AND CHARACTERIZATION OF BOVINE SERUM ALBUMIN USING CHROMATOGRAPHIC METHOD (SHORT COMMUNICATION)

Author(s)

Keywords

BOVINE SERUM ALBUMIN (BSA)

CHROMATOGRAPHY

IMMUNOAFFINITY PURIFICATION

POLYCLONAL ANTIBODY

WESTERN BLOTTING

Abstract

Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody.Methods: The POLYCLONAL ANTIBODY was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification, the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum. WESTERN BLOTTING analysis was used for functional assessment of immunoaffinity purified BSA.Results: The titer of anti-bovine albumin determined by ELISA was obtained 1: 256000. The SDS-PAGE showed up to 98% purity of isolated BSA and WESTERN BLOTTING confirmed the BSA functionality. Purified bovine serum albumin by affinity CHROMATOGRAPHY showed a single band with molecular weight of 66 KDa.Conclusion: Affinity CHROMATOGRAPHY using produced rabbit anti-BSA antibody would be an economical and safe method for purification of BSA.

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APA: Copy

balkani, Sanaz, Shamekhi, Sara, Raoufinia, Ramin, Parvan, Reza, & ABDOLALIZADEH, JALAL. (2016). PURIFICATION AND CHARACTERIZATION OF BOVINE SERUM ALBUMIN USING CHROMATOGRAPHIC METHOD (SHORT COMMUNICATION). ADVANCED PHARMACEUTICAL BULLETIN, 6(4), 651-654. SID. https://sid.ir/paper/331851/en

Vancouver: Copy

balkani Sanaz, Shamekhi Sara, Raoufinia Ramin, Parvan Reza, ABDOLALIZADEH JALAL. PURIFICATION AND CHARACTERIZATION OF BOVINE SERUM ALBUMIN USING CHROMATOGRAPHIC METHOD (SHORT COMMUNICATION). ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2016;6(4):651-654. Available from: https://sid.ir/paper/331851/en

IEEE: Copy

Sanaz balkani, Sara Shamekhi, Ramin Raoufinia, Reza Parvan, and JALAL ABDOLALIZADEH, “PURIFICATION AND CHARACTERIZATION OF BOVINE SERUM ALBUMIN USING CHROMATOGRAPHIC METHOD (SHORT COMMUNICATION),” ADVANCED PHARMACEUTICAL BULLETIN, vol. 6, no. 4, pp. 651–654, 2016, [Online]. Available: https://sid.ir/paper/331851/en

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