ESTIMATION OF GROUP B STREPTOCOCCUS COLONIZATION IN HIGH-RISK NEONATES BY PCR AND STANDARD CULTURE

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SHIRVANI FARIBA; SHAHROCHI NADER; RADFAR MITRA; LAKESTANI DAVOOD

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Journal Paper

Paper Information

Journal: Archives of Pediatric Infectious Diseases Year:2014 | Volume:2 | Issue:4 Page(s): 1-5

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Title

ESTIMATION OF GROUP B STREPTOCOCCUS COLONIZATION IN HIGH-RISK NEONATES BY PCR AND STANDARD CULTURE

Author(s)

SHIRVANI FARIBA | SHAHROCHI NADER | RADFAR MITRA | LAKESTANI DAVOOD | Issue Writer Certificate

Keywords

STREPTOCOCCUS AGALACTIAE

INFANT

NEWBORN

COLONIZATION

Abstract

Background: Group B Streptococcus (GBS) (STREPTOCOCCUS AGALACTIAE) is the leading cause of morbidity and mortality of NEWBORN INFANTs considered a leading factor causing septicemia after birth. The standard method for the diagnosis of GBS COLONIZATION is culture in a selective medium, but PCR has a high sensitivity and specificity.Objectives: The goal of this study was to estimate the COLONIZATION of GBS in rectum of neonates of high-risk mothers by culture and PCR method.Patients and Methods: Samples were taken from rectal mucosa of 154 neonatesof high-risk mothers for GBS by swabs. Samples were tested by standard culture using Todd Hewitt broth and blood agar and also by PCR using primers specific for cfb gene.Results: Of 154 neonates, Culture identified 17 (11%) neonates as colonized by GBS, and the PCR assay could identify 27 (17%) neonates with positive results for GBS. Mothers age range was 17-40 years (mean=26.1±5.1). Maternal age was significantly lower in PCR positive group (P=0.038) and in culture positive group (P=0.015). Using culture as the gold standard, sensitivity, NPV, specificity, and PPV of PCR were 100%, 100%, 92%, and 62%, respectively. The time required for PCR assay and culture were 2hours and 36hours, respectively.Conclusions: This study showed that the incidence of GBS in Iranian high-risk neonates is high, so we strongly recommend screening of high-risk neonates for detection of GBS.

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APA: Copy

SHIRVANI, FARIBA, SHAHROCHI, NADER, RADFAR, MITRA, & LAKESTANI, DAVOOD. (2014). ESTIMATION OF GROUP B STREPTOCOCCUS COLONIZATION IN HIGH-RISK NEONATES BY PCR AND STANDARD CULTURE. ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES, 2(4), 1-5. SID. https://sid.ir/paper/331978/en

Vancouver: Copy

SHIRVANI FARIBA, SHAHROCHI NADER, RADFAR MITRA, LAKESTANI DAVOOD. ESTIMATION OF GROUP B STREPTOCOCCUS COLONIZATION IN HIGH-RISK NEONATES BY PCR AND STANDARD CULTURE. ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES[Internet]. 2014;2(4):1-5. Available from: https://sid.ir/paper/331978/en

IEEE: Copy

FARIBA SHIRVANI, NADER SHAHROCHI, MITRA RADFAR, and DAVOOD LAKESTANI, “ESTIMATION OF GROUP B STREPTOCOCCUS COLONIZATION IN HIGH-RISK NEONATES BY PCR AND STANDARD CULTURE,” ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES, vol. 2, no. 4, pp. 1–5, 2014, [Online]. Available: https://sid.ir/paper/331978/en

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