MEDIP REAL-TIME QPCR HAS THE POTENTIAL FOR NONINVASIVE PRENATAL SCREENING OF FETAL TRISOMY 21

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KAZEMI MOHAMMAD; SALEHI MANSOOR; KHEIROLLAHI MAJID

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Journal Paper

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Journal: International Journal of Molecular and Cellular Medicine Year:2017 | Volume:6 | Issue:1 Page(s): 13-21

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Title

MEDIP REAL-TIME QPCR HAS THE POTENTIAL FOR NONINVASIVE PRENATAL SCREENING OF FETAL TRISOMY 21

Author(s)

KAZEMI MOHAMMAD | SALEHI MANSOOR | KHEIROLLAHI MAJID | Issue Writer Certificate

Keywords

DOWN SYNDROME

TRISOMY 21

PRENATAL DIAGNOSIS

CELL-FREE FETAL DNA; NONINVASIVE PRENATAL TESTING

PRENATAL GENETIC SCREENING

Abstract

This study aimed to verify the reliability of the 7 tissue differentially methylated regions used in the methylated DNA immunoprecipitation (MeDIP) real- time quantitative polymerase chain reaction (real-time qPCR) based approach of fetal DNA in maternal blood to diagnosis of fetal TRISOMY 21. Forty pregnant women with high risk pregnancy who were referred after first or second trimester screening tests, were selected randomly. For each sample whole DNA extraction (mother and fetus), fragmentation of DNA, immunoprecipitation of methylated DNA and real- time qPCR using 7 primer pairs was performed. D-value for each sample was calculated using the following formula D=-4.908+0.254 XEP1+0.409 XEP4+0.793 XEP5+0.324 XEP6+0.505 XEP7+0.508 XEP9+ 0.691 XEP12. In all normal cases, D value was negative, while it was positive in all trisomy cases. Therefore, all normal and TRISOMY 21 cases were classified correctly which correspond to 100% specificity and 100% sensitivity for this method. The MeDIP real-time qPCR method has provided the opportunity for noninvasive PRENATAL DIAGNOSIS of fetal TRISOMY 21 to be potentially employed into the routine practice of diagnostic laboratories.

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APA: Copy

KAZEMI, MOHAMMAD, SALEHI, MANSOOR, & KHEIROLLAHI, MAJID. (2017). MEDIP REAL-TIME QPCR HAS THE POTENTIAL FOR NONINVASIVE PRENATAL SCREENING OF FETAL TRISOMY 21. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 6(1), 13-21. SID. https://sid.ir/paper/332185/en

Vancouver: Copy

KAZEMI MOHAMMAD, SALEHI MANSOOR, KHEIROLLAHI MAJID. MEDIP REAL-TIME QPCR HAS THE POTENTIAL FOR NONINVASIVE PRENATAL SCREENING OF FETAL TRISOMY 21. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE[Internet]. 2017;6(1):13-21. Available from: https://sid.ir/paper/332185/en

IEEE: Copy

MOHAMMAD KAZEMI, MANSOOR SALEHI, and MAJID KHEIROLLAHI, “MEDIP REAL-TIME QPCR HAS THE POTENTIAL FOR NONINVASIVE PRENATAL SCREENING OF FETAL TRISOMY 21,” INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, vol. 6, no. 1, pp. 13–21, 2017, [Online]. Available: https://sid.ir/paper/332185/en

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