RAPID INDUCTION OF NEURAL DIFFERENTIATION IN HUMAN UMBILICAL CORD MATRIX MESENCHYMAL STEM CELLS BY CAMP-ELEVATING AGENTS

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SHAHBAZI ATEFEH; SAFA MAJID; ALIKARAMI FATEMEH; KARGOZAR SAEID; ASADI MOHAMMAD HOSSEIN; JOGHATAEI MOHAMMAD TAGHI; SOLEIMANI MANSOOREH

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Journal: International Journal of Molecular and Cellular Medicine Year:2016 | Volume:5 | Issue:3 Page(s): 167-177

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Title

RAPID INDUCTION OF NEURAL DIFFERENTIATION IN HUMAN UMBILICAL CORD MATRIX MESENCHYMAL STEM CELLS BY CAMP-ELEVATING AGENTS

Author(s)

Keywords

UMBILICAL CORD MATRIX MESENCHYMAL STEM CELLS

NEURAL DIFFERENTIATION

CAMP

Abstract

Human umbilical cord matrix (hUCM) is considered as a promising source of mesenchymal stem cells (MSCs) due to several advantages over other tissues. The potential of NEURAL DIFFERENTIATION of hUCM-MSCs is of great interest in the context of treating neurodegenerative diseases. In recent years, considerable efforts have been made to establishin vitro conditions for improving the differentiation of hUCM-MSCs toward neuronal cells. In the present study, we evaluated the NEURAL DIFFERENTIATION potential of hUCM-MSCs in the presence of CAMPelevating agents forskolin and 3-isobutyl-1-methylxanthine (IBMX). hUCM-MSCs were isolated from fetal umbilical cord and characterized by flow cytometry analysis for mesenchymal specific markers. Mesodermal differentiation potential was assessed through selective media with lineage-specific induction factors. For assessment of NEURAL DIFFERENTIATION, cells were cultured in the presence of CAMP-elevating agents for 8 and 24 h. The neuronal differentiated MSCs were characterized for neuronal specific markers by immunocytochemistry and western blotting. Isolated hUCM-MSCs were found positive for mesenchymal markers (CD73, CD90, and CD105) while negative for hematopoietic markers (CD34 and CD45). Following neural induction, most cells represented neural-like cells morphology. Neural markers including-tubulin III (Tuj-1), neuron-specific enolase (NSE), microtubule-associated protein-2 (MAP-2) and nestin were expressed in treated cells with respect to control group. The astrocyte specific marker, glial fibrillary acidic protein (GFAP) was also shown by immunofluorescence in treated cells. (These findings demonstrate that hUCM-MSCs have the ability to rapidly differentiate into neural cell types of neuron-like cells and astrocytes by CAMP-elevating agents without the presence of growth factors.

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APA: Copy

SHAHBAZI, ATEFEH, SAFA, MAJID, ALIKARAMI, FATEMEH, KARGOZAR, SAEID, ASADI, MOHAMMAD HOSSEIN, JOGHATAEI, MOHAMMAD TAGHI, & SOLEIMANI, MANSOOREH. (2016). RAPID INDUCTION OF NEURAL DIFFERENTIATION IN HUMAN UMBILICAL CORD MATRIX MESENCHYMAL STEM CELLS BY CAMP-ELEVATING AGENTS. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 5(3), 167-177. SID. https://sid.ir/paper/332215/en

Vancouver: Copy

SHAHBAZI ATEFEH, SAFA MAJID, ALIKARAMI FATEMEH, KARGOZAR SAEID, ASADI MOHAMMAD HOSSEIN, JOGHATAEI MOHAMMAD TAGHI, SOLEIMANI MANSOOREH. RAPID INDUCTION OF NEURAL DIFFERENTIATION IN HUMAN UMBILICAL CORD MATRIX MESENCHYMAL STEM CELLS BY CAMP-ELEVATING AGENTS. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE[Internet]. 2016;5(3):167-177. Available from: https://sid.ir/paper/332215/en

IEEE: Copy

ATEFEH SHAHBAZI, MAJID SAFA, FATEMEH ALIKARAMI, SAEID KARGOZAR, MOHAMMAD HOSSEIN ASADI, MOHAMMAD TAGHI JOGHATAEI, and MANSOOREH SOLEIMANI, “RAPID INDUCTION OF NEURAL DIFFERENTIATION IN HUMAN UMBILICAL CORD MATRIX MESENCHYMAL STEM CELLS BY CAMP-ELEVATING AGENTS,” INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, vol. 5, no. 3, pp. 167–177, 2016, [Online]. Available: https://sid.ir/paper/332215/en

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