Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

180
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

121
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Differentiation Potential of Nestin (+) and Nestin (-) Cells Derived from Human Bone Marrow Mesenchymal Stem Cells into Functional Insulin Producing Cells

Pages

  1-13

Abstract

 The feasibility of isolating and manipulating mesenchymal stem cells (MSCs) from human patients provides hope for curing numerous diseases and disorders. Recent phenotypic analysis has shown heterogeneity of MSCs. Nestin progenitor cell is a subpopulation within MSCs which plays a role in pancreas regeneration during embryogenesis. This study aimed to separate nestin (+) cells from human bone marrow MSCs, and differentiate these cells into functional insulin producing cells (IPCs) compared with nestin (-) cells. Manual magnetic separation was performed to obtain nestin (+) cells from MSCs. Approximately 91± 3. 3% of nestin (+) cells were positive for anti-nestin antibody. Pluripotent genes were overexpressed in nestin (+) cells compared with nestin (-) cells as revealed by quantitative real time-PCR (qRT-PCR). Following in vitro differentiation, flow cytometric analysis showed that 2. 7± 0. 5% of differentiated nestin (+) cells were positive for anti-insulin antibody in comparison with 0. 08± 0. 02% of nestin (-) cells. QRT-PCR showed higher expression of insulin and other endocrine genes in comparison with nestin (-) cells. While immunofluorescence technique showed the presence of insulin and C-peptide granules in nestin (+) cells. Therefore, our results introduced nestin (+) cells as a pluripotent subpopulation within human MSCs which is capable to differentiate and produce functional IPCs.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Rashed, Sahar, Gabr, Mahmoud, Abdel Aziz, Abdel Aziz, Zakaria, Mahmoud, Khater, Sherry, Ismail, Amani, Fouad, Ali, & Refaie, Ayman. (2019). Differentiation Potential of Nestin (+) and Nestin (-) Cells Derived from Human Bone Marrow Mesenchymal Stem Cells into Functional Insulin Producing Cells. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 8(1), 1-13. SID. https://sid.ir/paper/332249/en

    Vancouver: Copy

    Rashed Sahar, Gabr Mahmoud, Abdel Aziz Abdel Aziz, Zakaria Mahmoud, Khater Sherry, Ismail Amani, Fouad Ali, Refaie Ayman. Differentiation Potential of Nestin (+) and Nestin (-) Cells Derived from Human Bone Marrow Mesenchymal Stem Cells into Functional Insulin Producing Cells. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE[Internet]. 2019;8(1):1-13. Available from: https://sid.ir/paper/332249/en

    IEEE: Copy

    Sahar Rashed, Mahmoud Gabr, Abdel Aziz Abdel Aziz, Mahmoud Zakaria, Sherry Khater, Amani Ismail, Ali Fouad, and Ayman Refaie, “Differentiation Potential of Nestin (+) and Nestin (-) Cells Derived from Human Bone Marrow Mesenchymal Stem Cells into Functional Insulin Producing Cells,” INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, vol. 8, no. 1, pp. 1–13, 2019, [Online]. Available: https://sid.ir/paper/332249/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top