Migration Gene Expression of Human Umbilical Cord Mesenchymal Stem Cells: A Comparison between Monophosphoryl Lipid A and Supernatant of Lactobacillus acidophilus

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SHOJAEIAN ALI; Mehri Ghahfarrokhi Ameneh; BANITALEBI DEHKORDI MEHDI

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Journal Paper

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Journal: International Journal of Molecular and Cellular Medicine Year:2019 | Volume:8 | Issue:2 Page(s): 154-161

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Title

Migration Gene Expression of Human Umbilical Cord Mesenchymal Stem Cells: A Comparison between Monophosphoryl Lipid A and Supernatant of Lactobacillus acidophilus

Author(s)

SHOJAEIAN ALI | Mehri Ghahfarrokhi Ameneh | BANITALEBI DEHKORDI MEHDI | Issue Writer Certificate

Keywords

Human umbilical cord mesenchymal stem cells

monophosphoryl lipid A

Lactobacillus acidophilus

VCAM-1

Abstract

The capacity of Human umbilical cord mesenchymal stem cells (hUMSCs) for migration and homing is very important in regenerative medicine. A detoxified derivative of lipopolysaccharides (LPS) that lacks many of the endotoxic properties of LPS is monophosphoryl lipid A (MPLA). Effects of MPLA on the induction of MSCs migration, have not yet been studied. Also, studies have shown that supernatant of Lactobacillus acidophilus (SLA) culture medium, can stimulate the proliferation of macrophages and lymphocytes in vitro by affecting the properties of the chemotaxis and angiogenesis. Our present study aimed to understanding of the migration of hUMSCs during treatment with MPLA and SLA, separately. HUMSCs were isolated from human umbilical cord and were characterized by investigating surface markers (CD105, CD90, anti-CD29, CD45, and CD34) and their differentiation into adipogenic and osteogenic lineages. HUMSCs were treated with MPLA (10-3 μ g/ml) and SLA (3 μ l/ml). The morphological changes were not observed in both treated MSCs. Expression levels of migration markers were determined by quantitative reverse transcription PCR analysis on 2, 4, 6 days after treatment. Results showed that VEGF and MMP-2 but not CXCR-4 was increased in the presence of both treatments. Also, SLA led to a decrease and increase of the expression of VLA-4 and VCAM-1, respectively, while MPLA increased both VLA-4 and VCAM-1 expression. Therefore, it can be suggested that MPLA has more prominent results than SLA, but both treatments can probably be considered as an inducing factor in migration.

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APA: Copy

SHOJAEIAN, ALI, Mehri Ghahfarrokhi, Ameneh, & BANITALEBI DEHKORDI, MEHDI. (2019). Migration Gene Expression of Human Umbilical Cord Mesenchymal Stem Cells: A Comparison between Monophosphoryl Lipid A and Supernatant of Lactobacillus acidophilus. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 8(2), 154-161. SID. https://sid.ir/paper/332253/en

Vancouver: Copy

SHOJAEIAN ALI, Mehri Ghahfarrokhi Ameneh, BANITALEBI DEHKORDI MEHDI. Migration Gene Expression of Human Umbilical Cord Mesenchymal Stem Cells: A Comparison between Monophosphoryl Lipid A and Supernatant of Lactobacillus acidophilus. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE[Internet]. 2019;8(2):154-161. Available from: https://sid.ir/paper/332253/en

IEEE: Copy

ALI SHOJAEIAN, Ameneh Mehri Ghahfarrokhi, and MEHDI BANITALEBI DEHKORDI, “Migration Gene Expression of Human Umbilical Cord Mesenchymal Stem Cells: A Comparison between Monophosphoryl Lipid A and Supernatant of Lactobacillus acidophilus,” INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, vol. 8, no. 2, pp. 154–161, 2019, [Online]. Available: https://sid.ir/paper/332253/en

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