Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro

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Rembe Julian Dario; Boehm Julia Katharina; Fromm Dornieden Carolin; Hauer Nina; Stuermer Ewa Klara

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Journal: International Journal of Molecular and Cellular Medicine Year:2020 | Volume:9 | Issue:2 Page(s): 165-179

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Title

Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro

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Keywords

Wound healing

zinc

fibroblasts

keratinocytes

antimicrobial efficacy

cell proliferation

apoptosis

Abstract

zinc as therapeutic agent in skin and wound care has been known for centuries, but its role is controversial and comprehensive investigations in nutrient-deficient environments are lacking. We aimed to provide a broad analysis of different zinc derivatives on proliferation, apoptosis and antimicrobial properties in a simulated nutrient-deficient environment in vitro. Human fibroblasts (CRL2522) and keratinocytes (HaCaT) were treated with a broad concentration range (10 – 0. 0001 μ g/mL) of zinc-sulfate (ZnSO4),-gluconate (ZnGluc) and-histidine (ZnHis) for 1-6 days under nutrient-deficient media conditions. cell proliferation was investigated by XTT assay. Targeted analyzes in proliferation (E2F1, PCNA) and apoptosis (TP53) associated genes were performed via qRT-PCR and apoptosis was determined via FACS (annexin V/7-AAD staining). antimicrobial efficacy was investigated using a quantitative suspension method against S. aureus, P. aeruginosa, E. coli, and C. albicans. The results indicated that 0. 1 to 0. 001 μ g/mL Zn increased cell proliferation in both cell lines. fibroblasts were more susceptible with significant proliferation peaks on days 2 & 6, and days 1 & 4 for keratinocytes. No relevant changes in gene expression were detected for E2F1 and PCNA nor for TP53. Annexin-V/7-AAD-staining of fibroblasts revealed a small, yet insignificant reduction of apoptosis induction for ZnGluc and ZnSO4. ZnGluc and ZnSO4 (0. 1%) achieved high microbial reductions (4-5 log10 reductions) against tested pathogens. ZnGluc and ZnSO4 showed relevant pro-proliferative and antimicrobial, as well as tendential anti-apoptotic features in a simulated nutrient-deficient microenvironment in vitro. This further validates a potential benefit of local zinc treatment in deficient wound microenvironments.

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APA: Copy

Rembe, Julian Dario, Boehm, Julia Katharina, Fromm Dornieden, Carolin, Hauer, Nina, & Stuermer, Ewa Klara. (2020). Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, 9(2), 165-179. SID. https://sid.ir/paper/332289/en

Vancouver: Copy

Rembe Julian Dario, Boehm Julia Katharina, Fromm Dornieden Carolin, Hauer Nina, Stuermer Ewa Klara. Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro. INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE[Internet]. 2020;9(2):165-179. Available from: https://sid.ir/paper/332289/en

IEEE: Copy

Julian Dario Rembe, Julia Katharina Boehm, Carolin Fromm Dornieden, Nina Hauer, and Ewa Klara Stuermer, “Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro,” INTERNATIONAL JOURNAL OF MOLECULAR AND CELLULAR MEDICINE, vol. 9, no. 2, pp. 165–179, 2020, [Online]. Available: https://sid.ir/paper/332289/en

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