مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

HOW TO PREPARE BIOLOGICAL SAMPLES AND LIVE TISSUES FOR SCANNING ELECTRON MICROSCOPY (SEM) (REVIEW ARTICLE)

Pages

  63-80

Keywords

SCANNING ELECTRON MICROSCOPY (SEM) 

Abstract

 In this article we review the application and procedures involved in scanning electron microscope (SEM) to observe biological and LIVE TISSUES through using SEM at high resolution. We discuss practical methods for optimizing tissue preservation to achieve the two principal goals of biological specimen preparation: (a) preserving biological structures as close to their living configuration as possible, and (b) rendering them visible with the desired imaging method. We also review and discuss the relative merits of different fixing (chemical fixation and cryofixation), drying (air-drying, critical point-drying, freeze-drying and chemical-drying) and coating procedures of biological specimens with metals to facilitate visualization in the SEM.

Cites

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  • Cite

    APA: Copy

    MEHDIZADEH KASHI, ABOLFAZL, TAHERMANESH, KOBRA, CHAICHIAN, SHAHLA, JOGHATAEI, MOHAMMAD TAGHI, MORADI, FATEMEH, TAVANGAR, SEYED MOHAMMAD, MOUSAVI NAJAFABADI, ASHRAF SADAT, LOTFIBAKHSHAIESH, NASRIN, POUR BEYRANVAND, SHAHRAM, FAZEL ANVARI YAZDI, ABBAS, & ABED, SEYEDEH MEHR. (2014). HOW TO PREPARE BIOLOGICAL SAMPLES AND LIVE TISSUES FOR SCANNING ELECTRON MICROSCOPY (SEM) (REVIEW ARTICLE). GALEN MEDICAL JOURNAL, 3(2), 63-80. SID. https://sid.ir/paper/332584/en

    Vancouver: Copy

    MEHDIZADEH KASHI ABOLFAZL, TAHERMANESH KOBRA, CHAICHIAN SHAHLA, JOGHATAEI MOHAMMAD TAGHI, MORADI FATEMEH, TAVANGAR SEYED MOHAMMAD, MOUSAVI NAJAFABADI ASHRAF SADAT, LOTFIBAKHSHAIESH NASRIN, POUR BEYRANVAND SHAHRAM, FAZEL ANVARI YAZDI ABBAS, ABED SEYEDEH MEHR. HOW TO PREPARE BIOLOGICAL SAMPLES AND LIVE TISSUES FOR SCANNING ELECTRON MICROSCOPY (SEM) (REVIEW ARTICLE). GALEN MEDICAL JOURNAL[Internet]. 2014;3(2):63-80. Available from: https://sid.ir/paper/332584/en

    IEEE: Copy

    ABOLFAZL MEHDIZADEH KASHI, KOBRA TAHERMANESH, SHAHLA CHAICHIAN, MOHAMMAD TAGHI JOGHATAEI, FATEMEH MORADI, SEYED MOHAMMAD TAVANGAR, ASHRAF SADAT MOUSAVI NAJAFABADI, NASRIN LOTFIBAKHSHAIESH, SHAHRAM POUR BEYRANVAND, ABBAS FAZEL ANVARI YAZDI, and SEYEDEH MEHR ABED, “HOW TO PREPARE BIOLOGICAL SAMPLES AND LIVE TISSUES FOR SCANNING ELECTRON MICROSCOPY (SEM) (REVIEW ARTICLE),” GALEN MEDICAL JOURNAL, vol. 3, no. 2, pp. 63–80, 2014, [Online]. Available: https://sid.ir/paper/332584/en

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