MIR-155 EFFECTIVELY INDUCES APOPTOSIS IN K562 PHILADELPHIA POSITIVE CELL LINE THROUGH UPREGULATION OF P27KIP1

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EDALATI FATHABAD MAHDI; KARIMIPOUR MORTEZA; ALIZADEH SHABAN; ABDOLI ASGHAR; ATASHI AMIR; SAYADI MAHTAB

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Journal: BioImpacts Year:2017 | Volume:7 | Issue:2 Page(s): 109-114

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Title

MIR-155 EFFECTIVELY INDUCES APOPTOSIS IN K562 PHILADELPHIA POSITIVE CELL LINE THROUGH UPREGULATION OF P27KIP1

Author(s)

Keywords

APOPTOSIS

K562

MIR-155

PHILADELPHIA

P27KIP1

Abstract

Introduction: Chronic myelogenous leukemia (CML) is a myeloproliferative disorder caused by the PHILADELPHIA chromosome translocation, at (9; 22), which results in BCR-ABL fusion tyrosine kinase oncoprotein. This fusion induces down-regulation of MIR-155. Upregulation of MIR-155 can influence cell fate via the effect on P27KIP1 and APOPTOSIS. The aim of this study was to induce APOPTOSIS in K562 CML cell line by overexpression of miR- 155.Methods: The K562 cell line was transfected with pLenti-III-pre mir155-GFP constructs through electroporation. Then, overexpression of MIR-155 as well as the expression level of P27KIP1 and c-Myc was analyzed by quantitative PCR (qPCR). The level of p27 (Kip1) protein expression was measured by Western blot and the Annexin V method was carried out to investigate APOPTOSIS.Results: Flow cytometric analysis results of K562 cells transfected with pLenti-III-pre mir155- GFP construct showed a significant increase in cell APOPTOSIS. Gene expression and protein level of P27KIP1 were upregulated. However, there was no change in c-Myc expression profile.Conclusion: MIR-155 could be a promising approach to aid in the treatment of CML. However, further studies are required in this respect.

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References

PERCEPTION OF SIDE EFFECTS OF CHEMOTHERAPY AMONG CANCER PATIENTS IN B.P. KOIRALA MEMORIAL CANCER HOSPITAL BHARATPUR, NEPAL

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APA: Copy

EDALATI FATHABAD, MAHDI, KARIMIPOUR, MORTEZA, ALIZADEH, SHABAN, ABDOLI, ASGHAR, ATASHI, AMIR, & SAYADI, MAHTAB. (2017). MIR-155 EFFECTIVELY INDUCES APOPTOSIS IN K562 PHILADELPHIA POSITIVE CELL LINE THROUGH UPREGULATION OF P27KIP1. BIOIMPACTS, 7(2), 109-114. SID. https://sid.ir/paper/334079/en

Vancouver: Copy

EDALATI FATHABAD MAHDI, KARIMIPOUR MORTEZA, ALIZADEH SHABAN, ABDOLI ASGHAR, ATASHI AMIR, SAYADI MAHTAB. MIR-155 EFFECTIVELY INDUCES APOPTOSIS IN K562 PHILADELPHIA POSITIVE CELL LINE THROUGH UPREGULATION OF P27KIP1. BIOIMPACTS[Internet]. 2017;7(2):109-114. Available from: https://sid.ir/paper/334079/en

IEEE: Copy

MAHDI EDALATI FATHABAD, MORTEZA KARIMIPOUR, SHABAN ALIZADEH, ASGHAR ABDOLI, AMIR ATASHI, and MAHTAB SAYADI, “MIR-155 EFFECTIVELY INDUCES APOPTOSIS IN K562 PHILADELPHIA POSITIVE CELL LINE THROUGH UPREGULATION OF P27KIP1,” BIOIMPACTS, vol. 7, no. 2, pp. 109–114, 2017, [Online]. Available: https://sid.ir/paper/334079/en

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