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Information Journal Paper

Title

SURFACE PLASMON RESONANCE AND MOLECULAR DOCKING STUDIES OF BOVINE SERUM ALBUMIN INTERACTION WITH NEOMYCIN: KINETIC AND THERMODYNAMIC ANALYSIS

Pages

  91-97

Keywords

EQUILIBRIUM CONSTANTS (KD) 

Abstract

 Introduction: The interactions between biomacromolecules such as serum albumin (SA) and various drugs have attracted increasing research attention in recent years. However, the study of SA with those drugs that have relatively high hydrophilicity and a lower affinity for SA could be a challenging issue. At the present study, the interaction of bovine SA (BSA) with neomycin as a hydrophilic drug has been investigated using SURFACE PLASMON RESONANCE (SPR) and molecular docking methods.Methods: BSA was immobilized on the carboxymethyl dextran hydrogel sensor chip after activation of carboxylic groups through NHS/EDC and, then, the neomycin interaction with BSA at different concentrations (1-128 mM) was investigated.Results: Dose-response sensorgrams of BSA upon increasing concentration of neomycin has been shown through SPR analysis. The small KD value (4.96 e-7 at 40oC) demonstrated high affinity of neomycin to BSA. THERMODYNAMIC parameters were calculated through van’t Hoff equation at 4 different temperatures. The results showed that neomycin interacts with BSA via Van der Waals interactions and hydrogen bonds and increase of KD with temperature rising indicated that the binding process was ENTROPY driven. Molecular docking study confirmed that hydrogen bond was the major intermolecular force stabilizing neomycin-BSA complex.Conclusion: The attained results showed that neomycin molecules can efficiently distribute within the body after interaction with BSA in spite of having hydrophilic properties. Besides, SPR can be considered as a useful instrument for study of the interaction of hydrophilic drugs with SA.

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    APA: Copy

    SHARIFI, MARYAM, EZZATI NAZHAD DOLATABADI, JAFAR, FATHI, FARZANEH, Zakariazadeh, Mostafa, BARZEGAR, ABOLFAZL, RASHIDI, MOHAMMAD, TAJALLI, HABIB, & RASHIDI, MOHAMMAD REZA. (2017). SURFACE PLASMON RESONANCE AND MOLECULAR DOCKING STUDIES OF BOVINE SERUM ALBUMIN INTERACTION WITH NEOMYCIN: KINETIC AND THERMODYNAMIC ANALYSIS. BIOIMPACTS, 7(2), 91-97. SID. https://sid.ir/paper/334081/en

    Vancouver: Copy

    SHARIFI MARYAM, EZZATI NAZHAD DOLATABADI JAFAR, FATHI FARZANEH, Zakariazadeh Mostafa, BARZEGAR ABOLFAZL, RASHIDI MOHAMMAD, TAJALLI HABIB, RASHIDI MOHAMMAD REZA. SURFACE PLASMON RESONANCE AND MOLECULAR DOCKING STUDIES OF BOVINE SERUM ALBUMIN INTERACTION WITH NEOMYCIN: KINETIC AND THERMODYNAMIC ANALYSIS. BIOIMPACTS[Internet]. 2017;7(2):91-97. Available from: https://sid.ir/paper/334081/en

    IEEE: Copy

    MARYAM SHARIFI, JAFAR EZZATI NAZHAD DOLATABADI, FARZANEH FATHI, Mostafa Zakariazadeh, ABOLFAZL BARZEGAR, MOHAMMAD RASHIDI, HABIB TAJALLI, and MOHAMMAD REZA RASHIDI, “SURFACE PLASMON RESONANCE AND MOLECULAR DOCKING STUDIES OF BOVINE SERUM ALBUMIN INTERACTION WITH NEOMYCIN: KINETIC AND THERMODYNAMIC ANALYSIS,” BIOIMPACTS, vol. 7, no. 2, pp. 91–97, 2017, [Online]. Available: https://sid.ir/paper/334081/en

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