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Information Journal Paper

Title

The Inhibitory Effects of Lactobacillus Reuteri’ s Cell Wall on Cell Proliferation in the HCT-116 Colorectal Cancer Cell Line

Pages

  126-130

Abstract

 Background: Colorectal cancer (CRC) is the third and a second common cancer in men and women respectively in the world and about 1. 4 million new cases diagnosed in 2012. The normal gut microflora consists of bacterial species. One group of them is probiotics, which confer a health benefit to the host. Lactobacillus reuteri (L. reuteri) is known as a probiotic, which lead to the prevention of Colorectal cancer. The aim of this study was to assess the inhibitory effects of Lactobacillus reuteri’ s cell wall on cell proliferation in the Colorectal cancer HCT-116 cell line. Materials and Methods: The cells of HCT-116 cell line were grown at 37° C, 5% CO2. L. reuteri was obtained from the Iranian Biological Resource Center and cultured in the MRS Broth at 37° C for 48h anaerobically. The cell wall was prepared by the freezing-thawing procedure. So the inhibitory effect of L. reuteri on the growth and proliferation of HCT-116 cells was assessed by MTT assay. Results: The cell wall from L. reuteri inhibited cell proliferation on Colorectal cancer HCT-116 cell line. It showed dose-and – time dependent inhibition. Conclusion: These results demonstrated that cell wall of L. reuteri inhibits cell proliferation of HCT-116 cell line.

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    APA: Copy

    Rafiei Atani, Zahra, FAGHIHLOO, EBRAHIM, GHALAVAND, ZOHREH, & ESLAMI, GITA. (2018). The Inhibitory Effects of Lactobacillus Reuteri’ s Cell Wall on Cell Proliferation in the HCT-116 Colorectal Cancer Cell Line. NOVELTY IN BIOMEDICINE, 6(3), 126-130. SID. https://sid.ir/paper/336675/en

    Vancouver: Copy

    Rafiei Atani Zahra, FAGHIHLOO EBRAHIM, GHALAVAND ZOHREH, ESLAMI GITA. The Inhibitory Effects of Lactobacillus Reuteri’ s Cell Wall on Cell Proliferation in the HCT-116 Colorectal Cancer Cell Line. NOVELTY IN BIOMEDICINE[Internet]. 2018;6(3):126-130. Available from: https://sid.ir/paper/336675/en

    IEEE: Copy

    Zahra Rafiei Atani, EBRAHIM FAGHIHLOO, ZOHREH GHALAVAND, and GITA ESLAMI, “The Inhibitory Effects of Lactobacillus Reuteri’ s Cell Wall on Cell Proliferation in the HCT-116 Colorectal Cancer Cell Line,” NOVELTY IN BIOMEDICINE, vol. 6, no. 3, pp. 126–130, 2018, [Online]. Available: https://sid.ir/paper/336675/en

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