Identification of E. coli O157: H7 by Using Specific Primers for rfbE and stx2b Genes

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAKHSHI M.; EBRAHIMI F.; NAZARIAN SH.; Tarverdizade y.; SADEGHI D.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian South Medical Journal Year:2017 | Volume:20 | Issue:3 Page(s): 267-277

Download Full-Text

Persian Verion

View:

835

Download:

Cites:

Information Journal Paper

Title

Identification of E. coli O157: H7 by Using Specific Primers for rfbE and stx2b Genes

Author(s)

Keywords

Identification of E.coli O157:H7 strainQ1

polymerase chain reactionQ2

rfbE geneQ1

stx2b geneQ1

Abstract

Background: E. coli O157: H7 is one of the intestinal pathogens which causes serious lesion in gastrointestinal system. Detection of this bacteria that able to produce toxin and is the major responsible for hospital infection, usually done by culture on sorbitol-MacConkey agar which is time-consuming test. The aim of this study was the preparation a fast and precise method in order to identification of this bacterium by molecular method based on PCR technique. Material and Methods: rfbE and stx2b genes were selected for proprietary identification of E. coli O157: H7 and the amplification of them were performed by PCR following designing specific primers. Sorbitol-MacConkey agar was used to verification of growth ability of selected colonies during PCR. Results: By appearance of the bonds belong to rfbE and stx2b genes on agarose gel, the ability of designed primers in gene detection in samples of E. coli O157: H7 was verified. Colonies which selected during PCR have growth potency on sorbitol-MacConkey agar medium. Conclusion: It was revealed that we can prepare a fast, precise and relative comfortable method for detection of E. coli O157: H7 strain by using PCR technique and specific primers than other available methods.

Cites

No record.

References

No record.

Cite

APA: Copy

BAKHSHI, M., EBRAHIMI, F., NAZARIAN, SH., Tarverdizade, y., & SADEGHI, D.. (2017). Identification of E. coli O157: H7 by Using Specific Primers for rfbE and stx2b Genes. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 20(3 ), 267-277. SID. https://sid.ir/paper/33814/en

Vancouver: Copy

BAKHSHI M., EBRAHIMI F., NAZARIAN SH., Tarverdizade y., SADEGHI D.. Identification of E. coli O157: H7 by Using Specific Primers for rfbE and stx2b Genes. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2017;20(3 ):267-277. Available from: https://sid.ir/paper/33814/en

IEEE: Copy

M. BAKHSHI, F. EBRAHIMI, SH. NAZARIAN, y. Tarverdizade, and D. SADEGHI, “Identification of E. coli O157: H7 by Using Specific Primers for rfbE and stx2b Genes,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 20, no. 3 , pp. 267–277, 2017, [Online]. Available: https://sid.ir/paper/33814/en

Related Journal Papers