ASSESSMENT OF HEMOLYTICAL ACTIVITY OF PROTEINS FROM TENTACLES OF SEA ANEMONE, STICHODACTYLA HADDONI

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BAHARLOUEI M.; KHODABANDEH S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Iranian South Medical Journal Year:2016 | Volume:19 | Issue:1 Page(s): 27-36

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Title

ASSESSMENT OF HEMOLYTICAL ACTIVITY OF PROTEINS FROM TENTACLES OF SEA ANEMONE, STICHODACTYLA HADDONI

Author(s)

BAHARLOUEI M. | KHODABANDEH S. | Issue Writer Certificate

Keywords

SEA ANEMONEQ2

STICHODACTYLA HADDONIQ1

CYTOLYSINQ1

HEMOLYTIC ACTIVITYQ1

Abstract

Background: SEA ANEMONE is ocean dwelling and typically organisms sessile. These tentacles produce a variety of peptides and proteins that act as, neurotoxins and CYTOLYSINs toxins. CYTOLYSINs due to their effect on specific tissue and lysing properties are known as antiparasitic or antitumor compounds.Materials and Methods: SEA ANEMONE STICHODACTYLA HADDONI were collected from the coast of Hormuz Island in November 2012 and tentacles extraction was performed by using of saline PBS solvents. Peptides above 10kDa and peptides below 10kDa were separated by amicon® Ultra-15 10K device. The HEMOLYTIC ACTIVITY was assessed by the micro hemolytic method for human, rainbow trout Oncorhynchus mykiss and common carp Cyprinus carpio erythrocytes.Results: Uniform red color suspension in the wells considered as positive hemolysis. The crude extracts, peptides above 10kDa and peptides below 10kDa at various concentrations were showed hemolytic effect on human, common carp Cyprinus carpioand rainbow trout Oncorhynchus mykiss erythrocytes.Conclusion: The crude extract, peptides above 10kDa and peptides below 10kDa have a hemolytic effect on human erythrocytes and two species of fish. The venoms showed the most hemolytic activities on human blood among others.

Cites

INTERACTION BETWEEN SORBITOL AND HEN EGG WHITE LYSOZYME: FROM DYNAMICS TO STRUCTURE

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APA: Copy

BAHARLOUEI, M., & KHODABANDEH, S.. (2016). ASSESSMENT OF HEMOLYTICAL ACTIVITY OF PROTEINS FROM TENTACLES OF SEA ANEMONE, STICHODACTYLA HADDONI. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 19(1), 27-36. SID. https://sid.ir/paper/33895/en

Vancouver: Copy

BAHARLOUEI M., KHODABANDEH S.. ASSESSMENT OF HEMOLYTICAL ACTIVITY OF PROTEINS FROM TENTACLES OF SEA ANEMONE, STICHODACTYLA HADDONI. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2016;19(1):27-36. Available from: https://sid.ir/paper/33895/en

IEEE: Copy

M. BAHARLOUEI, and S. KHODABANDEH, “ASSESSMENT OF HEMOLYTICAL ACTIVITY OF PROTEINS FROM TENTACLES OF SEA ANEMONE, STICHODACTYLA HADDONI,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 19, no. 1, pp. 27–36, 2016, [Online]. Available: https://sid.ir/paper/33895/en

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