EFFECTIVE IN VITRO GENE DELIVERY TO MURINE CANCEROUS BRAIN CELLS USING CARBON NANOTUBE-POLYETHYLENIMINE CONJUGATES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HASHEM NIA AZADEH; ESHGHI HOSSEIN; ABNOUS KHALIL; RAMEZANI MOHAMMAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NANOMEDICINE JOURNAL Year:2015 | Volume:2 | Issue:2 Page(s): 129-140

Download Full-Text

View:

295

Download:

103

Cites:

Information Journal Paper

Title

EFFECTIVE IN VITRO GENE DELIVERY TO MURINE CANCEROUS BRAIN CELLS USING CARBON NANOTUBE-POLYETHYLENIMINE CONJUGATES

Author(s)

HASHEM NIA AZADEH | ESHGHI HOSSEIN | ABNOUS KHALIL | RAMEZANI MOHAMMAD | Issue Writer Certificate

Keywords

CARBON NANOTUBE

DISULFIDE BOND

FUNCTIONALIZATION

GENE DELIVERY

POLYETHYLENEIMINE

Abstract

Objective (s): CARBON NANOTUBE (CNT) has been widely applied at molecular and cellular levels due to its exceptional properties. Studies based on conjugation of CNTs with biological molecules indicated that biological activity is preserved. Polyethylenimine (PEI) is explored in designing novel GENE DELIVERY vectors due to its ability to condense plasmid DNA through electrostatic attraction. In this study FUNCTIONALIZATION and grafting polyethylenimine onto the surface of CARBON NANOTUBE was used to improve the solubility and biocompatibility.Materials and Methods: The effect of molecular weight of polymer on final efficacy of vectors has been investigated using three different molecular weights of polymer. In this study no linker was used and both segments (PEI and CNT) were directly attached resulted in the synthesis of three different vectors. Synthesized vectors were tested for their ability to condense plasmid DNA and cellular toxicity using ethidium bromide and MTT assays. Size and Zeta potential of nanoparticles was determined using Malvern zeta sizer. Evaluation of transfection efficiency of vectors was carried out on N2A cell line by different methods including qualitative fluorescence imaging, flow cytometry and luciferase assay.Results: All three synthesized vectors bear positive surface charges with sizes in the range of 85-190 nm. More than 80 percent of treated cells were viable and in the case of V25 significant improvement in reducing cytotoxicity compared to unmodified polymer was observed. Obtained results indicated that vector containing PEI 1.8 kDa has the greatest improvement in terms of its transfection efficiency compared to unmodified polymer.Conclusion: Conjugation of PEI with CARBON NANOTUBE les to new vectors with lowered cytotoxicity and higher transfection efficiency. The highest transfection efficiency was obtained with the lowest molecular weight PEI.

Cites

No record.

References

No record.

Cite

APA: Copy

HASHEM NIA, AZADEH, ESHGHI, HOSSEIN, ABNOUS, KHALIL, & RAMEZANI, MOHAMMAD. (2015). EFFECTIVE IN VITRO GENE DELIVERY TO MURINE CANCEROUS BRAIN CELLS USING CARBON NANOTUBE-POLYETHYLENIMINE CONJUGATES. NANOMEDICINE JOURNAL, 2(2), 129-140. SID. https://sid.ir/paper/341580/en

Vancouver: Copy

HASHEM NIA AZADEH, ESHGHI HOSSEIN, ABNOUS KHALIL, RAMEZANI MOHAMMAD. EFFECTIVE IN VITRO GENE DELIVERY TO MURINE CANCEROUS BRAIN CELLS USING CARBON NANOTUBE-POLYETHYLENIMINE CONJUGATES. NANOMEDICINE JOURNAL[Internet]. 2015;2(2):129-140. Available from: https://sid.ir/paper/341580/en

IEEE: Copy

AZADEH HASHEM NIA, HOSSEIN ESHGHI, KHALIL ABNOUS, and MOHAMMAD RAMEZANI, “EFFECTIVE IN VITRO GENE DELIVERY TO MURINE CANCEROUS BRAIN CELLS USING CARBON NANOTUBE-POLYETHYLENIMINE CONJUGATES,” NANOMEDICINE JOURNAL, vol. 2, no. 2, pp. 129–140, 2015, [Online]. Available: https://sid.ir/paper/341580/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops