CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING CFP-10 AND ESAT-6 GENES AND THEIR POTENTIAL IN LYMPHOCYTE PROLIFERATION

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TORABI AZAM; TAHMOORESPOUR MOJTABA; VAHEDI FATEMEH; MOSAVARI NADER; NASSIRI MOHAMMADREZA

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Paper Information

Journal: REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY Year:2013 | Volume:2 | Issue:1 Page(s): 0-0

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Title

CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING CFP-10 AND ESAT-6 GENES AND THEIR POTENTIAL IN LYMPHOCYTE PROLIFERATION

Author(s)

Keywords

MYCOBACTERIUM BOVIS

DNA VACCINE

ESAT-6

CFP-10

PPD

PROLIFERATION ASSAY

BALB/C MICE

Abstract

Background: Mycobacterium (M.) bovis is the agent of bovine tuberculosis (TB) in a range of animal species, including humans. Recent advances in immunology and the molecular biology of Mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new TB vaccine. The ESAT-6 and CFP-10 proteins of M. bovis are important structural and functional proteins known to be important immunogens.Methods: In the current study, the DNAs encoding these genes were utilized in the construction of pcDNA 3.1+/ESAT-6 and pcDNA3.1+/CFP-10 plasmids. After intramuscular injection of BALB/C MICE with these plasmids, ESAT-6 and CFP-10 mRNA expression was assessed by RT-PCR. Mice were inoculated and boosted with the plasmids to evaluate their effects on lymphocyte proliferation.Results: Our results indicate the plasmids are expressed at the RNA level and can induce lymphocyte proliferation.Conclusion: Further study is needed to characterize the effect of these antigens on the immune system and determine whether they are effective vaccine candidates against M. bovis.

Cites

DESIGNING AND CONSTRUCTION PCDNA3.1 VECTOR ENCODING CFP10 GENE OF MYCOBACTERIUM TUBERCULOSIS

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APA: Copy

TORABI, AZAM, TAHMOORESPOUR, MOJTABA, VAHEDI, FATEMEH, MOSAVARI, NADER, & NASSIRI, MOHAMMADREZA. (2013). CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING CFP-10 AND ESAT-6 GENES AND THEIR POTENTIAL IN LYMPHOCYTE PROLIFERATION. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2(1), 0-0. SID. https://sid.ir/paper/343125/en

Vancouver: Copy

TORABI AZAM, TAHMOORESPOUR MOJTABA, VAHEDI FATEMEH, MOSAVARI NADER, NASSIRI MOHAMMADREZA. CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING CFP-10 AND ESAT-6 GENES AND THEIR POTENTIAL IN LYMPHOCYTE PROLIFERATION. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2013;2(1):0-0. Available from: https://sid.ir/paper/343125/en

IEEE: Copy

AZAM TORABI, MOJTABA TAHMOORESPOUR, FATEMEH VAHEDI, NADER MOSAVARI, and MOHAMMADREZA NASSIRI, “CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING CFP-10 AND ESAT-6 GENES AND THEIR POTENTIAL IN LYMPHOCYTE PROLIFERATION,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 2, no. 1, pp. 0–0, 2013, [Online]. Available: https://sid.ir/paper/343125/en

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