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Information Journal Paper

Title

In vitro Delivery of HIV-1 Nef Antigen by Histidine-rich nona-arginine and Latarcin 1 peptide

Pages

  107-115

Abstract

 Introduction: The Nef accessory protein is an attractive antigenic candidate in the development of HIV-1 DNA-or protein-based vaccines. The most crucial disadvantage of DNA and protein-based vaccines is their low immunogenicity, which can be improved by Cell-penetrating peptides (CPPs) as effective carrier molecules. Methods: In this study, the HIV-1 Nef protein was generated in the Escherichia coli expression system for in vitro delivery using a novel CPP, Latarcin 1 peptide, in a non-covalent manner. Also, the Histidine-rich nona-arginine peptide was utilized to transfer the HIV-1 Nef gene. The size, morphology, and zeta potential of the complexes were evaluated by scanning electron microscopy (SEM) and Zetasizer. The efficiency of cell Transfection was studied using a fluorescence microscopy and flow cytometry for the DNA/CPP complexes and western blot analysis for the protein/CPP complexes. Results: The Nef protein generated in the BL21 strain migrated as a dominant band of ~30 kDa in SDS-PAGE. The SEM data confirmed the formation of stable complexes with a size below 200 nm. MTT assay demonstrated that the complexes did not represent any considerable cytotoxic effect compared to untreated HEK-293T cells. The results of fluorescence microscopy, flow cytometry, and western blotting revealed that the Nef DNA and protein constructs could be significantly transfected into HEK-293T cell line using these CPPs. Conclusion: These data suggest that the Histidine-rich nonaarginine peptide and Latarcin 1 peptide as CPPs can be considered as a promising approach in the development of the HIV-1 vaccine for gene or protein delivery.

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    Cite

    APA: Copy

    NAMAZI, FATEMEH, BOLHASSANI, AZAM, SADAT, SEYED MEHDI, & IRANI, SHIVA. (2019). In vitro Delivery of HIV-1 Nef Antigen by Histidine-rich nona-arginine and Latarcin 1 peptide. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, 7(4), 107-115. SID. https://sid.ir/paper/343224/en

    Vancouver: Copy

    NAMAZI FATEMEH, BOLHASSANI AZAM, SADAT SEYED MEHDI, IRANI SHIVA. In vitro Delivery of HIV-1 Nef Antigen by Histidine-rich nona-arginine and Latarcin 1 peptide. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES[Internet]. 2019;7(4):107-115. Available from: https://sid.ir/paper/343224/en

    IEEE: Copy

    FATEMEH NAMAZI, AZAM BOLHASSANI, SEYED MEHDI SADAT, and SHIVA IRANI, “In vitro Delivery of HIV-1 Nef Antigen by Histidine-rich nona-arginine and Latarcin 1 peptide,” JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, vol. 7, no. 4, pp. 107–115, 2019, [Online]. Available: https://sid.ir/paper/343224/en

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