Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Khalili Samani Maryam; BARATI MAHMOOD; Mir mohammadsadegh Navid; AMIN MOHSEN; SAMADI KUCHAK SARAEI ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES Year:2019 | Volume:7 | Issue:4 Page(s): 127-131

Download Full-Text

View:

177

Download:

Cites:

Information Journal Paper

Title

Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran

Author(s)

Keywords

Burns

Pseudomonas aeruginosa

Drug Resistance

Amino Acid Sequence

Point Mutation

Abstract

Introduction: Pseudomonas aeruginosa is an opportunistic pathogen of clinical importance, particularly in immunocompromised and burn patients. This bacterium is becoming resistant to many antibiotics via intrinsic or acquired mechanisms. Mutations in anti-mutator genes, such as pfpI, can be a potential intrinsic mechanism of antibiotic resistance. This study aimed to evaluate the possible effects of mutations of this gene on coding proteins of multi-drug resistant P. aeruginosa isolates. Methods: The antibiotic resistance pattern of 50 P. aeruginosa isolates against 9 anti-pseudomonas antibiotics was determined by the disk diffusion method. PCR, followed by sequencing, detected the mutations in the pfpI gene. The retrieved sequences were translated to the corresponding Amino Acid Sequences using an online protein database. The Amino Acid Sequences in mutated isolates were compared with the reference sequence using a multiple alignment method. Results: Out of 50 isolates, 43 (86%) were resistant to all antibiotics. Sequencing and multiple alignment analyses showed that amino acids in positions 21, 24, and 57 of pfpI gene were changed in resistant isolates, and all these mutations were observed in each isolate. Homology modeling showed that these amino acids were part of a cleft on the protease. The other Point Mutations resulted in amino acid changes were in positions 67, 83, and 165. Conclusion: The data obtained in this study showed that the pfpI gene of P. aeruginosa might have a significant effect on response to antibiotics. Further epidemiologic and comprehensive studies are required to confirm these findings.

Cites

No record.

References

No record.

Cite

APA: Copy

Khalili Samani, Maryam, BARATI, MAHMOOD, Mir mohammadsadegh, Navid, AMIN, MOHSEN, & SAMADI KUCHAK SARAEI, ALI. (2019). Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, 7(4), 127-131. SID. https://sid.ir/paper/343227/en

Vancouver: Copy

Khalili Samani Maryam, BARATI MAHMOOD, Mir mohammadsadegh Navid, AMIN MOHSEN, SAMADI KUCHAK SARAEI ALI. Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES[Internet]. 2019;7(4):127-131. Available from: https://sid.ir/paper/343227/en

IEEE: Copy

Maryam Khalili Samani, MAHMOOD BARATI, Navid Mir mohammadsadegh, MOHSEN AMIN, and ALI SAMADI KUCHAK SARAEI, “Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran,” JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, vol. 7, no. 4, pp. 127–131, 2019, [Online]. Available: https://sid.ir/paper/343227/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops