Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants

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MALEKAN MOHAMMADALI; SIADAT SEYED DAVAR; Aghasadeghi Mohammadreza; SHAHROKHI NADER; EYBPOOSH SANA; Afshari Elnaz; MOUSAVI SEYED FAZLOLLAH

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Journal: VACCINE RESEARCH Year:2019 | Volume:6 | Issue:2 Page(s): 37-41

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Title

Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants

Author(s)

Keywords

Streptococcus pneumonia

Meningococcal OMV

PhtD

Adjuvant

Abstract

Introduction: Streptococcus pneumoniae causes invasive and non-invasive diseases in children and adults. Currently, there are two types of pneumococcal vaccines: 23-valent pneumococcal polysaccharide vaccine and 13-valent pneumococcal conjugate vaccine which have caused many failures. Therefore, a new generation of pneumococcal vaccines is being pursued. Methods: An improved version of our previous study was performed using recombinant C-terminal of pneumococcal polyhistidine triad protein D (PhtD-C) as a vaccine antigen. The antigen was combined with meningococcal outer membrane vesicle (OMV) and alum as Adjuvants to immunize BALB/c mice intraperitoneally. The generated total IgG, specific IgG, IgG1 and IgG2a antibodies and the killing ability of pneumococci by an opsonophagocytosis assay were then assayed. Results: Immunization by 30 μ g PhtD-C and 50 μ g OMV as an Adjuvant, induced higher amounts of functional antibodies compared to our previous study while killing 50-55% of pneumococci cells. Conclusion: At optimized concentrations, PhtD-C and Meningococcal OMV could be considered as a potent immunogens and the induced specific IgGs were effective and functional for killing pneumococci.

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APA: Copy

MALEKAN, MOHAMMADALI, SIADAT, SEYED DAVAR, Aghasadeghi, Mohammadreza, SHAHROKHI, NADER, EYBPOOSH, SANA, Afshari, Elnaz, & MOUSAVI, SEYED FAZLOLLAH. (2019). Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants. VACCINE RESEARCH, 6(2), 37-41. SID. https://sid.ir/paper/344447/en

Vancouver: Copy

MALEKAN MOHAMMADALI, SIADAT SEYED DAVAR, Aghasadeghi Mohammadreza, SHAHROKHI NADER, EYBPOOSH SANA, Afshari Elnaz, MOUSAVI SEYED FAZLOLLAH. Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants. VACCINE RESEARCH[Internet]. 2019;6(2):37-41. Available from: https://sid.ir/paper/344447/en

IEEE: Copy

MOHAMMADALI MALEKAN, SEYED DAVAR SIADAT, Mohammadreza Aghasadeghi, NADER SHAHROKHI, SANA EYBPOOSH, Elnaz Afshari, and SEYED FAZLOLLAH MOUSAVI, “Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants,” VACCINE RESEARCH, vol. 6, no. 2, pp. 37–41, 2019, [Online]. Available: https://sid.ir/paper/344447/en

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