DETERMINATION OF PHYLOGENETIC GROUPS OF ESCHERICHIA COLI ISOLATED FROM HUMAN URINE IN URMIA CITY

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BAPONI SHAHIN; TARAVATI AFSHIN; DILMAGANI MEHDI

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Journal Paper

Paper Information

Journal: Crescent Journal of Medical and Biological Sciences Year:2016 | Volume:3 | Issue:3 Page(s): 97-99

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Information Journal Paper

Title

DETERMINATION OF PHYLOGENETIC GROUPS OF ESCHERICHIA COLI ISOLATED FROM HUMAN URINE IN URMIA CITY

Author(s)

BAPONI SHAHIN | TARAVATI AFSHIN | DILMAGANI MEHDI | Issue Writer Certificate

Keywords

URINARY TRACT INFECTION

ESCHERICHIA COLI

PHYLOGENETIC TYPING

M-PCR

Abstract

Objective: ESCHERICHIA COLI is the member of Enterobacteriaceae family and is one of the most important and common species of Escherichia in medicine. ESCHERICHIA COLI has high potential in creating different intestinal and extraintestinal diseases in human and animals. The goal of this research determination of phylogenetic groups of ESCHERICHIA COLI isolated from human urine in Urmia city.Materials and Methods: In the present research, 950 urine samples were studied from hospitalized patients in Urmia city. The urine sample were inoculated on MacCankey agar and blood agar and incubated at 37oC. Positive urine cultures was identified by standard laboratory methods involving morphological characteristics and biochemical tests. To determine the phylogenetic group, multiplex-PCR (m-PCR) was used. Primers used in this study could successfully amplify genes, including yjaA, chuA and TSPE4.C2 with 211, 279 and 152 bp, respectively.Results: Fifty samples were positive after culture and bacterial isolation. Fifteen of isolates belong to group A (30%), 6 belong to group B1 (12%), 20 belong to group B2 (40%) and 9 of them belong to group D (18%).Conclusion: The high number of bacteria in group B2 as extra intestinal pathogenic agent, indicative of having a care about choosing a treatment for such infections.

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References

ANTIBIOTIC RESISTANCE PATTERN OF DIFFERENT ESCHERICHIA COLI PHYLOGENETIC GROUPS ISOLATED FROM HUMAN URINARY TRACT INFECTION AND AVIAN COLIBACILLOSIS

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APA: Copy

BAPONI, SHAHIN, TARAVATI, AFSHIN, & DILMAGANI, MEHDI. (2016). DETERMINATION OF PHYLOGENETIC GROUPS OF ESCHERICHIA COLI ISOLATED FROM HUMAN URINE IN URMIA CITY. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, 3(3), 97-99. SID. https://sid.ir/paper/344946/en

Vancouver: Copy

BAPONI SHAHIN, TARAVATI AFSHIN, DILMAGANI MEHDI. DETERMINATION OF PHYLOGENETIC GROUPS OF ESCHERICHIA COLI ISOLATED FROM HUMAN URINE IN URMIA CITY. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES[Internet]. 2016;3(3):97-99. Available from: https://sid.ir/paper/344946/en

IEEE: Copy

SHAHIN BAPONI, AFSHIN TARAVATI, and MEHDI DILMAGANI, “DETERMINATION OF PHYLOGENETIC GROUPS OF ESCHERICHIA COLI ISOLATED FROM HUMAN URINE IN URMIA CITY,” CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, vol. 3, no. 3, pp. 97–99, 2016, [Online]. Available: https://sid.ir/paper/344946/en

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