DETERMINATION OF ADHESION ENCODING GENES OF UROPATHOGENIC ESCHERICHIA COLI

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BABY SABITHA; KARNAKER VIMAL KUMAR; GEETHA RISHIYUR KRISHNASWAMY

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Journal Paper

Paper Information

Journal: Avicenna Journal of Clinical Microbiology and Infection Year:2018 | Volume:5 | Issue:2 Page(s): 20-26

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Title

DETERMINATION OF ADHESION ENCODING GENES OF UROPATHOGENIC ESCHERICHIA COLI

Author(s)

BABY SABITHA | KARNAKER VIMAL KUMAR | GEETHA RISHIYUR KRISHNASWAMY | Issue Writer Certificate

Keywords

UROPATHOGENIC E. COLI

PAP

SFA

AFA GENES

ADHESINS

COMMENSAL E.COLI

Abstract

Background: The bacterial strains which cause symptomatic urinary tract infections possess diverse distinctive properties that enable them to overcome local host defences. The present study was undertaken to screen for the virulence factors of uropathogenic Escherichia coli (UPFC) by molecular methods and to detect the genotypic pathogenicity related sequences PAP, SFA, and AFA GENES in E. coli strains by polymerase chain reaction (PCR).Methods: A total of 350 E.coli isolates were obtained from symptomatic cases of urinary tract infections (UTI) and 60 E. coli faecal isolates were collected from apparently healthy individuals (during February 2014-2016) from outpatient department of a tertiary care hospital in Kerala, India. Isolates were tested for adhesive genes PAP, SFA, and afa.Results: Among the UPEC samples, 255 (72.85%) of the isolates were from females and 95 (27.14%) of them were obtained from males. The prevalence of PAP gene was seen in 72 of the total isolates which were the highest. The SFA genes were shown by 56 isolates. The AFA GENES were also in 7 of the isolates. Diversity of the UPEC associated virulence genes were established in this study.Conclusions: The E.coli causing UTI had greater percentage of virulence associated adhesin genes compared to E.coli from control stool samples.

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APA: Copy

BABY, SABITHA, KARNAKER, VIMAL KUMAR, & GEETHA, RISHIYUR KRISHNASWAMY. (2018). DETERMINATION OF ADHESION ENCODING GENES OF UROPATHOGENIC ESCHERICHIA COLI. AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION, 5(2), 20-26. SID. https://sid.ir/paper/346860/en

Vancouver: Copy

BABY SABITHA, KARNAKER VIMAL KUMAR, GEETHA RISHIYUR KRISHNASWAMY. DETERMINATION OF ADHESION ENCODING GENES OF UROPATHOGENIC ESCHERICHIA COLI. AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION[Internet]. 2018;5(2):20-26. Available from: https://sid.ir/paper/346860/en

IEEE: Copy

SABITHA BABY, VIMAL KUMAR KARNAKER, and RISHIYUR KRISHNASWAMY GEETHA, “DETERMINATION OF ADHESION ENCODING GENES OF UROPATHOGENIC ESCHERICHIA COLI,” AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION, vol. 5, no. 2, pp. 20–26, 2018, [Online]. Available: https://sid.ir/paper/346860/en

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