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Information Journal Paper

Title

EXPLORATION OF SPECIFIC DNA-BARCODES IN SHIGELLA DYSENTERIAE USING IN-SILICO ANALYSIS

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Abstract

 Background: SHIGELLA dysenteriae are Gram-negative and non-sporulating bacteria that cause illness in epithelial tissue of the colon and rectum. According to a preliminary analysis, rare or no reports could introduce highly reliable and specific genes, primers, and probes forS. dysenteriae recognition. Thus, it is necessary to detect specific genome parts in S. dysenteriae that could be used in diagnostic laboratories to recognize S. dysenteriae species confidently.Methods: Identification of specific S. dysenteriae genome regions as DNA-barcodes was the main objective of the current study to accrue detection of this species. To this end, S. dysenteriae genome was compared with other ENTEROBACTERIACEAE genomes.Results: Results indicated that there is little genetic distance between S. dysenteriae and E. COLI, and most of the genes are common between these 2 species. The lowest genome fluidity was observed betweenS. dysenteriae and Escherichia coli, and Salmonella enterica.Furthermore, the largest number of orthologous genes was observed between S. dysenteriae and E. COLI (O157_H7). All previous markers and virulent genes were also evaluated in the current study. However, no specific DNA barcodes were identified among already identified genes. Additionally, all regions of S. dysenteriae genome were investigated in the current study using specific region identifier programs by comparison with other ENTEROBACTERIACEAE strains.Conclusions: Finally, eight specific DNA-barcodes were identified in the current study that could be beneficial for specific recognition of S. dysenteriae strains.

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    APA: Copy

    KAMALI, MEHDI, BAKHSHI, BEHNAM, SALIMI, ALI, MOHSENI FARD, EHSAN, DARVISHI, MOHAMMAD HASAN, & EHGHAGHI, ELAHE. (2017). EXPLORATION OF SPECIFIC DNA-BARCODES IN SHIGELLA DYSENTERIAE USING IN-SILICO ANALYSIS. AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION, 4(4), 0-0. SID. https://sid.ir/paper/346874/en

    Vancouver: Copy

    KAMALI MEHDI, BAKHSHI BEHNAM, SALIMI ALI, MOHSENI FARD EHSAN, DARVISHI MOHAMMAD HASAN, EHGHAGHI ELAHE. EXPLORATION OF SPECIFIC DNA-BARCODES IN SHIGELLA DYSENTERIAE USING IN-SILICO ANALYSIS. AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION[Internet]. 2017;4(4):0-0. Available from: https://sid.ir/paper/346874/en

    IEEE: Copy

    MEHDI KAMALI, BEHNAM BAKHSHI, ALI SALIMI, EHSAN MOHSENI FARD, MOHAMMAD HASAN DARVISHI, and ELAHE EHGHAGHI, “EXPLORATION OF SPECIFIC DNA-BARCODES IN SHIGELLA DYSENTERIAE USING IN-SILICO ANALYSIS,” AVICENNA JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTION, vol. 4, no. 4, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/346874/en

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