ISOLATION OFCLOSTRIDIUM DIFFICILE AND DETECTION OF A AND B TOXINS ENCODING GENES

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IMANI FOOLADI ABBAS ALI; RAHMATI SADEGH; FALLAH MEHRABADI JALIL; HALABIAN RAHELEH; SEDIGHIAN HAMID; Soltanpour Mohammad Javad; RAHIMI MOHSEN

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Journal: International Journal of Enteric Pathogens Year:2014 | Volume:2 | Issue:1 Page(s): 0-0

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Title

ISOLATION OFCLOSTRIDIUM DIFFICILE AND DETECTION OF A AND B TOXINS ENCODING GENES

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Keywords

CLOSTRIDIUM DIFFICILE

ENTEROCOLITIS

PSEUDOMEMBRANOUS

POLYMERASE CHAIN REACTION

Abstract

Background: CLOSTRIDIUM DIFFICILE is the most important anaerobic, gram positive, spore forming bacillus which is known as a prevalent factor leading to antibiotic associated diarrheas and is the causative agent of pseudomembrane colitis. The role of this bacterium along with the over use of antibiotics have been proved to result in colitis. The major virulence factors of these bacteria are the A and B toxins.Objectives: The purpose of this study was to isolate C. difficile from stool samples and detect A and B toxins encoding genes, in order to serve as a routine method for clinical diagnosis.Materials and Methods: Recognition of A and B toxins encoding genes by uniplex and multiplex PCR using two pairs of primers from 136 accumulated stool samples.Results: Results of the present study showed that out of 136 stool samples, three C. difficile were isolated and these strains contained A and B toxins encoding genes.Conclusions: It was concluded that although detection of C. difficile from stool samples based on PCR (polymerase chain reaction) is expensive, yet this method is more sensitive and less time-consuming than culture methods and can be used as a clinical laboratory test.

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APA: Copy

IMANI FOOLADI, ABBAS ALI, RAHMATI, SADEGH, FALLAH MEHRABADI, JALIL, HALABIAN, RAHELEH, SEDIGHIAN, HAMID, Soltanpour, Mohammad Javad, & RAHIMI, MOHSEN. (2014). ISOLATION OFCLOSTRIDIUM DIFFICILE AND DETECTION OF A AND B TOXINS ENCODING GENES. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 2(1), 0-0. SID. https://sid.ir/paper/347472/en

Vancouver: Copy

IMANI FOOLADI ABBAS ALI, RAHMATI SADEGH, FALLAH MEHRABADI JALIL, HALABIAN RAHELEH, SEDIGHIAN HAMID, Soltanpour Mohammad Javad, RAHIMI MOHSEN. ISOLATION OFCLOSTRIDIUM DIFFICILE AND DETECTION OF A AND B TOXINS ENCODING GENES. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2014;2(1):0-0. Available from: https://sid.ir/paper/347472/en

IEEE: Copy

ABBAS ALI IMANI FOOLADI, SADEGH RAHMATI, JALIL FALLAH MEHRABADI, RAHELEH HALABIAN, HAMID SEDIGHIAN, Mohammad Javad Soltanpour, and MOHSEN RAHIMI, “ISOLATION OFCLOSTRIDIUM DIFFICILE AND DETECTION OF A AND B TOXINS ENCODING GENES,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 2, no. 1, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/347472/en

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