COMPARING THE ACCURACY RATE OF TWO DIFFERENT UNIVERSAL PRIMERS IN ENTERIC PATHOGEN DIAGNOSIS FROM BLOOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLEIMANI ESMAEIL; HONARMAND HAMIDREZA; NIKOKAR IRAJ; FALAKIAN ZINAB

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Enteric Pathogens Year:2014 | Volume:2 | Issue:1 Page(s): 0-0

Download Full-Text

View:

224

Download:

111

Cites:

Information Journal Paper

Title

COMPARING THE ACCURACY RATE OF TWO DIFFERENT UNIVERSAL PRIMERS IN ENTERIC PATHOGEN DIAGNOSIS FROM BLOOD

Author(s)

SOLEIMANI ESMAEIL | HONARMAND HAMIDREZA | NIKOKAR IRAJ | FALAKIAN ZINAB | Issue Writer Certificate

Keywords

BACTEREMIA

PRISMA UNIVERSAL BOND 3 PRIMER

CULTURE

POLYMERASE CHAIN REACTION

Abstract

Background: Detecting enteric bacteria in blood by CULTURE is a slow assay with low accuracy rate. PCR might be a suitable alternative assay but as several species can cause BACTEREMIA, it is necessary to use universal primers.Objectives: In this study we evaluated and compared two pairs of universal primers in detecting four enteric bacteria in blood, which are common causes of BACTEREMIA in human.Materials and Methods: Standard strains of E. faecalis, S. typhi, E. coli, and S. Aeruginosa, were used in this study. A serially diluted bacterial suspension of all strains was made for inoculation to four sets of defibrinated sheep blood which were used to prepare blood specimens with different bacterial contents for performing routine assay and PCR. PCR was performed using two different universal primers designed from two ribosomal genes, 16sr RNA and 23sr RNA.Results: PCR with 16sr RNA universal primer showed more accuracy rate than both blood CULTURE and PCR with 23sr RNA universal primer.Mean time for performing PCR assay and blood CULTURE was eight and 48 hours, respectively.Conclusions: Both PCR with 16sr RNA and 23sr RNA universal primers have more accuracy rate than blood CULTURE and are faster in detection of BACTEREMIA. PCR with 16sr RNA universal primer is more accurate than both PCR with 16sr RNA universal primer and blood CULTURE for diagnosis of BACTEREMIA.

Cites

No record.

References

No record.

Cite

APA: Copy

SOLEIMANI, ESMAEIL, HONARMAND, HAMIDREZA, NIKOKAR, IRAJ, & FALAKIAN, ZINAB. (2014). COMPARING THE ACCURACY RATE OF TWO DIFFERENT UNIVERSAL PRIMERS IN ENTERIC PATHOGEN DIAGNOSIS FROM BLOOD. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 2(1), 0-0. SID. https://sid.ir/paper/347480/en

Vancouver: Copy

SOLEIMANI ESMAEIL, HONARMAND HAMIDREZA, NIKOKAR IRAJ, FALAKIAN ZINAB. COMPARING THE ACCURACY RATE OF TWO DIFFERENT UNIVERSAL PRIMERS IN ENTERIC PATHOGEN DIAGNOSIS FROM BLOOD. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2014;2(1):0-0. Available from: https://sid.ir/paper/347480/en

IEEE: Copy

ESMAEIL SOLEIMANI, HAMIDREZA HONARMAND, IRAJ NIKOKAR, and ZINAB FALAKIAN, “COMPARING THE ACCURACY RATE OF TWO DIFFERENT UNIVERSAL PRIMERS IN ENTERIC PATHOGEN DIAGNOSIS FROM BLOOD,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 2, no. 1, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/347480/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops