MOLECULAR CHARACTERIZATION OF FASCIOLA SAMPLES USING SEQUENCES OF SECOND INTERNAL TRANSCRIBED SPACER-RDNA IN DIFFERENT GEOGRAPHICAL LOCALITIES OF SISTAN AND BALOUCHESTAN PROVINCE, IRAN

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SHAHBAKHSH MAHSA; NABAVI REZA; GANJALI MARYAM

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Journal Paper

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Journal: International Journal of Enteric Pathogens Year:2016 | Volume:4 | Issue:1 Page(s): 49-54

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Title

MOLECULAR CHARACTERIZATION OF FASCIOLA SAMPLES USING SEQUENCES OF SECOND INTERNAL TRANSCRIBED SPACER-RDNA IN DIFFERENT GEOGRAPHICAL LOCALITIES OF SISTAN AND BALOUCHESTAN PROVINCE, IRAN

Author(s)

SHAHBAKHSH MAHSA | NABAVI REZA | GANJALI MARYAM | Issue Writer Certificate

Keywords

PCR

RDNA

SISTAN AND BALOUCHESTAN

ITS2

FASCIOLA

Abstract

Background: The FASCIOLA trematodes are the most common liver flukes, living in a range of animals with global distribution and resulting in profound economic loss and public health challenges. Previous studies have indicated that the sequences of the secondinternal transcribed spacer (ITS-2) of ribosomal DNA (rDNA) provide reliable genetic markers for molecular systemic studies of FASCIOLA.Objectives: The objective of the present study was to characterize FASCIOLA samples from different geographical regions of SISTAN AND BALOUCHESTAN province using sequences of second internal transcribed spacer (ITS-2) of ribosomal DNA (rDNA).Materials and Methods: Twenty adult trematodes were collected from the livers of slaughtered infected cattle. Total genomic DNAwas extracted and ITS-2 RDNA targets were amplified by polymerase chain reaction (PCR). All samples were sequenced and investigatedusing the ClustalW2 sequence alignment tool and MEGA software. The sequences of some Iranian and non-Iranian isolates were used forcomparison, in order to evaluate the variation in sequence homology between geographically different trematode populations.Results: The results of comparing the ITS-2 sequences with the BLAST GenBank database showed one type of sequence for F. hepatica andthree different types of sequences for F. gigantica in the specimens.Conclusions: The present study demonstrated that FASCIOLA samples from cattle in two geographical locations in SISTAN AND BALOUCHESTANprovince represented no genetic diversity in F. hepatica and high genetic variation in F. gigantica.

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APA: Copy

SHAHBAKHSH, MAHSA, NABAVI, REZA, & GANJALI, MARYAM. (2016). MOLECULAR CHARACTERIZATION OF FASCIOLA SAMPLES USING SEQUENCES OF SECOND INTERNAL TRANSCRIBED SPACER-RDNA IN DIFFERENT GEOGRAPHICAL LOCALITIES OF SISTAN AND BALOUCHESTAN PROVINCE, IRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 4(1), 49-54. SID. https://sid.ir/paper/347489/en

Vancouver: Copy

SHAHBAKHSH MAHSA, NABAVI REZA, GANJALI MARYAM. MOLECULAR CHARACTERIZATION OF FASCIOLA SAMPLES USING SEQUENCES OF SECOND INTERNAL TRANSCRIBED SPACER-RDNA IN DIFFERENT GEOGRAPHICAL LOCALITIES OF SISTAN AND BALOUCHESTAN PROVINCE, IRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2016;4(1):49-54. Available from: https://sid.ir/paper/347489/en

IEEE: Copy

MAHSA SHAHBAKHSH, REZA NABAVI, and MARYAM GANJALI, “MOLECULAR CHARACTERIZATION OF FASCIOLA SAMPLES USING SEQUENCES OF SECOND INTERNAL TRANSCRIBED SPACER-RDNA IN DIFFERENT GEOGRAPHICAL LOCALITIES OF SISTAN AND BALOUCHESTAN PROVINCE, IRAN,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 4, no. 1, pp. 49–54, 2016, [Online]. Available: https://sid.ir/paper/347489/en

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