PHENOTYPIC DETECTION OF ESBL, MBL (IMP-1), AND AMPC ENZYMES, AND THEIR COEXISTENCE INENTEROBACTER AND KLEBSIELLA SPECIES ISOLATED FROM CLINICAL SPECIMENS

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SHAHANDEH ZAHRA; SADIGHIAN FARAHNAZ; Rekabpour Khadijeh Beigom

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Journal Paper

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Journal: International Journal of Enteric Pathogens Year:2016 | Volume:4 | Issue:2 Page(s): 0-0

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Title

PHENOTYPIC DETECTION OF ESBL, MBL (IMP-1), AND AMPC ENZYMES, AND THEIR COEXISTENCE INENTEROBACTER AND KLEBSIELLA SPECIES ISOLATED FROM CLINICAL SPECIMENS

Author(s)

SHAHANDEH ZAHRA | SADIGHIAN FARAHNAZ | Rekabpour Khadijeh Beigom | Issue Writer Certificate

Keywords

KLEBSIELLA

ENTEROBACTER

AMPC BETA-LACTAMASE

Abstract

Background: Bacterial resistance to beta-lactam is a major problem in all developed and developing countries. The genera of KLEBSIELLA andEnterobacter are associated with opportunistic and nosocomial infections. All beta-lactamase genes can cause dissemination of resistance to beta-lactams.Objectives: The present studyaimedto investigate the phenotypic detection of beta-lactamases in ENTEROBACTER and KLEBSIELLA species isolated from clinical specimens.Materials and Methods: This cross-sectional study was performed on 59 KLEBSIELLA spp. and 49 ENTEROBACTER spp. isolated from clinical samples. They were confirmed using API 20E. These bacteria were evaluated for the production of extended-spectrum beta lactamase (ESBL), metallo-beta-lactamase (MBL) (IMP-1), and the pAmpC and iAmpC enzymes. This was done using the Clinical and Laboratory Standards Institute (CLSI) method, 2-mercaptopropionic acid, the cefoxitin method, and the use of imipenem as an enzyme inducer, respectively. Mask-ESBL production was also identified, using different concentrations of 3-amino-phenyl boronic acid compound. Data were analyzed with SPSS version 22.Results: In total, 38 (64.4%) KLEBSIELLA spp. and 41 (83.7%) ENTEROBACTER spp. produced at least one type of beta-lactamase. AmpCproducing ENTEROBACTERspp. (71.4%), and ESBL-producing KLEBSIELLA spp. (42.4%) had the highest prevalence of beta-lactamase types in each genus. There weretwobacteria in both types that were resistant to all antibiotics without producing any type of beta-lactamase.Conclusions: According to our findings, it is necessary to pay special attention to ESBL production in KLEBSIELLA spp., while in ENTEROBACTER spp., it is essential to search for AmpC production (chromosomal and plasmid). In addition, the genotypic evaluation of beta-lactamase variety in these bacteria may be necessary in different geographical areas.

Cites

IN VITRO INHIBITORY EFFECT OF ALCOHOLIC AND AQUEOUSEXTRACT OF VACCINIUM ARCTOSTAPHYLOS ON ESBLS PRODUCING KLEBSIELLA STRAINS ISOLATED FROM CLINICAL SPECIMENS IN KARAJ

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Cite

APA: Copy

SHAHANDEH, ZAHRA, SADIGHIAN, FARAHNAZ, & Rekabpour, Khadijeh Beigom. (2016). PHENOTYPIC DETECTION OF ESBL, MBL (IMP-1), AND AMPC ENZYMES, AND THEIR COEXISTENCE INENTEROBACTER AND KLEBSIELLA SPECIES ISOLATED FROM CLINICAL SPECIMENS. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 4(2), 0-0. SID. https://sid.ir/paper/347515/en

Vancouver: Copy

SHAHANDEH ZAHRA, SADIGHIAN FARAHNAZ, Rekabpour Khadijeh Beigom. PHENOTYPIC DETECTION OF ESBL, MBL (IMP-1), AND AMPC ENZYMES, AND THEIR COEXISTENCE INENTEROBACTER AND KLEBSIELLA SPECIES ISOLATED FROM CLINICAL SPECIMENS. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2016;4(2):0-0. Available from: https://sid.ir/paper/347515/en

IEEE: Copy

ZAHRA SHAHANDEH, FARAHNAZ SADIGHIAN, and Khadijeh Beigom Rekabpour, “PHENOTYPIC DETECTION OF ESBL, MBL (IMP-1), AND AMPC ENZYMES, AND THEIR COEXISTENCE INENTEROBACTER AND KLEBSIELLA SPECIES ISOLATED FROM CLINICAL SPECIMENS,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 4, no. 2, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/347515/en

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