مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

MOLECULAR TYPING OF KLEBSIELLA PNEUMONIAE ISOLATES USING REPETITIVE EXTRAGENIC PALINDROMIC SEQUENCE-BASED PCR IN A HOSPITAL IN TEHRAN, IRAN

Pages

  27-30

Abstract

 Background: The presence of extended-spectrum b-lactamases (ESBLs) is increasing worldwide and bla CTX-M1 is the predominant b-lactamase.Objective: This study was conducted to determine the ESBL production and prevalence of bla CTX-M1, bla SHV and bla TEM and AmpC genes and repetitive extragenic palindromic polymerase chain reaction (rep-PCR) pattern among KLEBSIELLA PNEUMONIAE isolates in Tehran from 2014 to 2016.Materials and Methods: One hundred eleven isolates were collected during the study period. The PCR was employed to detect the bla CTX-M1, bla SHV, bla TEM and AmpC genes. The genetic relation of isolates was performed using REP-PCR TYPING method.Results: Eighty-three and 86 isolates showed Minimum inhibitory concentration (MIC) ≥2 against ceftazidime and cefotaxime, respectively and 80 (72%) isolates exhibited ESBL production. The prevalence of bla CTX-M1, bla SHV, bla TEM and AmpC genes among ESBL producers was 92.5% (n=74), 66.2% (n=53), 56.2% (n=45) and 2.5% (n=2), respectively. The REP-PCR TYPING pattern of isolates showed a wide diversity, indicating the polyclonal spread of CTX-M type producing isolates.Conclusion: The findings of this study highlighted the emergence and spread of K. pneumoniae isolates producing CTX-M and other ESBL enzymes with diverse genetic backgrounds in a hospital in Tehran.

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    APA: Copy

    GHASEMIAN, ABDOLMAJID, SHAFIEI, MORVARID, ESLAMI, MAJID, VAFAEI, MAHTAB, NOJOOMI, FARSHAD, & RAJABI VARDANJANI, HASSAN. (2018). MOLECULAR TYPING OF KLEBSIELLA PNEUMONIAE ISOLATES USING REPETITIVE EXTRAGENIC PALINDROMIC SEQUENCE-BASED PCR IN A HOSPITAL IN TEHRAN, IRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 6(1), 27-30. SID. https://sid.ir/paper/347590/en

    Vancouver: Copy

    GHASEMIAN ABDOLMAJID, SHAFIEI MORVARID, ESLAMI MAJID, VAFAEI MAHTAB, NOJOOMI FARSHAD, RAJABI VARDANJANI HASSAN. MOLECULAR TYPING OF KLEBSIELLA PNEUMONIAE ISOLATES USING REPETITIVE EXTRAGENIC PALINDROMIC SEQUENCE-BASED PCR IN A HOSPITAL IN TEHRAN, IRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2018;6(1):27-30. Available from: https://sid.ir/paper/347590/en

    IEEE: Copy

    ABDOLMAJID GHASEMIAN, MORVARID SHAFIEI, MAJID ESLAMI, MAHTAB VAFAEI, FARSHAD NOJOOMI, and HASSAN RAJABI VARDANJANI, “MOLECULAR TYPING OF KLEBSIELLA PNEUMONIAE ISOLATES USING REPETITIVE EXTRAGENIC PALINDROMIC SEQUENCE-BASED PCR IN A HOSPITAL IN TEHRAN, IRAN,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 6, no. 1, pp. 27–30, 2018, [Online]. Available: https://sid.ir/paper/347590/en

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