مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

506
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

107
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

PURITY DETERMINING OF CULTURED OECS FROM OLFACTORY MUCOSA OF RATS' PUPS

Pages

  12-21

Abstract

 Introduction: Cell transplantation is one of the main strategies for SPINAL CORD INJURY repair. As OECS of the olfactory mucosa can be obtained by simple biopsy in all individuals without affecting their smell sensation, OECS considered as a promising candidate for autologous transplantation in the nervous system injury, especially for spinal cord repair. Thereby in the current study OECS were cultured from olfactory mucosa of 7 days old rats' pups and their purity was examined by flow-cytometry after simultaneous double staining for p75 and GFAP markers.Materials and methods: 7 days old Wistar rats' pups were deeply anesthetized by ketamine / xylazine (60/6mg/Kg). Then the nasal cavity was opened sagittally and the olfactory mucosa was separated from posterior part of nasal septum and at last OECS were obtained from lamina properia of olfactory mucosa and were cultured. The cultured cells were simultaneously immunolabeled for p75 and GFAP markers and finally purity of cultured cells assessed by flow-cytometry.Results: cultured OECS demonstrated two different morphologies, a spindle shape Schwann-like and an astrocyte-like OECS with flat sheet-like morphology. In addition, simultaneous immunolabeling for p75 and GFAP markers of OECS exhibited OECS were positive for both markers at the same time. The flow-cytometry results displayed that 87.9±2.4% of cells were p75/ GFAP double positive cells, 1.05±0.4 only p75 positive and 5.8±1.5% were single positive for GFAP.Conclusion: Purity of cultured OECS in our study is probably more than 87.9% by flow- owing to p75+/S100+and GFAP+/S100+olfactory ensheathing cells were not counted. Thus the culture procedure of this study seems to be a good protocol for OECS purifying and CELL THERAPY in CNS damages.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    JOGHATAEI, MOHAMMAD TAGHI, BAKHTIARI, MEHRDAD, FARAHMAND GHAVI, FARHID, ZANDI, MOJGAN, IMANI, MOHAMMAD, MOAIERI, ARDSHIR, & AZIZI, MONIREH. (2016). PURITY DETERMINING OF CULTURED OECS FROM OLFACTORY MUCOSA OF RATS' PUPS. JOURNALS OF BASIC RESEARCH IN MEDICAL SCIENCES, 3(4), 12-21. SID. https://sid.ir/paper/348468/en

    Vancouver: Copy

    JOGHATAEI MOHAMMAD TAGHI, BAKHTIARI MEHRDAD, FARAHMAND GHAVI FARHID, ZANDI MOJGAN, IMANI MOHAMMAD, MOAIERI ARDSHIR, AZIZI MONIREH. PURITY DETERMINING OF CULTURED OECS FROM OLFACTORY MUCOSA OF RATS' PUPS. JOURNALS OF BASIC RESEARCH IN MEDICAL SCIENCES[Internet]. 2016;3(4):12-21. Available from: https://sid.ir/paper/348468/en

    IEEE: Copy

    MOHAMMAD TAGHI JOGHATAEI, MEHRDAD BAKHTIARI, FARHID FARAHMAND GHAVI, MOJGAN ZANDI, MOHAMMAD IMANI, ARDSHIR MOAIERI, and MONIREH AZIZI, “PURITY DETERMINING OF CULTURED OECS FROM OLFACTORY MUCOSA OF RATS' PUPS,” JOURNALS OF BASIC RESEARCH IN MEDICAL SCIENCES, vol. 3, no. 4, pp. 12–21, 2016, [Online]. Available: https://sid.ir/paper/348468/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button