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Information Journal Paper

Title

Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats

Pages

  127-132

Abstract

 Introduction: Gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologue RF amide-related peptide (RFRP) are known to inhibit the secretion of gonadotropins. In addition, Substance P (SP), a member of tachykinin’ s family, can increase the firing Rate of kisspeptin/ neurokinin B/dynorphin (KNDy) neurons and provoke the secretion of gonadotropins. In this experimental study, we investigated the effects of co-administRation of RFRP-3 and SP on the expression of KISS-1 and GnRH genes, as gonadotropin regulator genes, in male Rats. Methods: Forty-two mature Wistar Rats were randomly allocated into 7 groups (n=6 in each group). Animals in each group intracerebroventricularly received either saline+DMSO, SP (1nmol), RFRP-3 (5 nmol), SP (1nmol) + RFRP-3 (5 nmol), SP (1 nmol) + RF9 (RFRP-3 receptor antagonist, 10 nmol), SP (1 nmol) + P234 (kisspeptin receptor antagonist, 1 nmol) + RFRP-3 (5 nmol), or SP (1 nmol) + CP-96, 345 (SP receptor antagonist, 5 nmol) + RFRP-3 (5 nmol). Two hours after injections, hypothalamic samples were collected to evaluate the expression of target genes by real-time PCR. Results: Injections in SP and SP + RF9 groups increased the expression of both GnRH and KISS-1 genes (P < 0. 05). Injections in RFRP-3 and SP + RFRP-3 + CP-96, 345 groups significantly decreased the expression of GnRH and KISS-1 genes (P < 0. 05). However, injections of SP + RFRP-3 and SP + RFRP-3 + P234 did not significantly change the expression of GnRH and KISS-1 genes. Conclusion: The results of our study indicated that SP antagonized the effects of RFRP-3 in the expression of hypothalamic KISS-1 and GnRH genes.

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    APA: Copy

    Rahdar, Parastoo, & KHAZALI, HOMAYOUN. (2018). Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats. INTERNATIONAL JOURNAL OF BASIC SCIENCE IN MEDICINE, 3(3), 127-132. SID. https://sid.ir/paper/350712/en

    Vancouver: Copy

    Rahdar Parastoo, KHAZALI HOMAYOUN. Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats. INTERNATIONAL JOURNAL OF BASIC SCIENCE IN MEDICINE[Internet]. 2018;3(3):127-132. Available from: https://sid.ir/paper/350712/en

    IEEE: Copy

    Parastoo Rahdar, and HOMAYOUN KHAZALI, “Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats,” INTERNATIONAL JOURNAL OF BASIC SCIENCE IN MEDICINE, vol. 3, no. 3, pp. 127–132, 2018, [Online]. Available: https://sid.ir/paper/350712/en

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