ELECTROCHEMICAL BEHAVIOR OF ADULT AND FETAL HEMOGLOBIN AT GOLD-COATED MAGNETIC IRON OXIDE NANOPARTICLE

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ESKANDARI KHADIJEH; GHOURCHIAN HEDAYATOLLAH; BANAEI AGHDAS

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Journal Paper

Paper Information

Journal: JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS Year:2017 | Volume:4 | Issue:3 Page(s): 669-674

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Information Journal Paper

Title

ELECTROCHEMICAL BEHAVIOR OF ADULT AND FETAL HEMOGLOBIN AT GOLD-COATED MAGNETIC IRON OXIDE NANOPARTICLE

Author(s)

ESKANDARI KHADIJEH | GHOURCHIAN HEDAYATOLLAH | BANAEI AGHDAS | Issue Writer Certificate

Keywords

BIOSENSOR

DIRECT ELECTROCHEMISTRY

H2O2

GOLD-COATED

IRON OXIDE NANOPARTICLES

HEMOGLOBIN

Abstract

A feasible and fast method for Adult HEMOGLOBIN (A-Hb) and Fetal HEMOGLOBIN (F-Hb) study was developed by immobilization of Hb on GOLD-COATED magnetic IRON OXIDE NANOPARTICLES (GMNPs).The prepared GMNPs composite nanoparticles with 60 nm diameter were used as a carrier for the immobilization of Hb. The A-Hb and F-Hb were physically attached to the GMNPs nanoparticles. The DIRECT ELECTROCHEMISTRY of F-Hb and A-Hb showed a quasi-reversible cyclic voltammogram corresponding to the Heme group with a formal potential of 314 and -334 mV in 0.1M PBS (pH 6.2), respectively. The apparent charge transfer rate constant (ks) and transfer coefficient (a) for electron transfer between the electrode surface and protein were calculated as 0.29/s and 0.1 for F-Hb and 0.21/s and 0.47 for A-Hb. The linear concentration rangesare17.3–225 and 7.4-53 mM for F-H band A-Hb BIOSENSORs for H2O2 detection. The lifetime of BIOSENSOR is more than 2 weeks.

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APA: Copy

ESKANDARI, KHADIJEH, GHOURCHIAN, HEDAYATOLLAH, & BANAEI, AGHDAS. (2017). ELECTROCHEMICAL BEHAVIOR OF ADULT AND FETAL HEMOGLOBIN AT GOLD-COATED MAGNETIC IRON OXIDE NANOPARTICLE. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, 4(3), 669-674. SID. https://sid.ir/paper/352938/en

Vancouver: Copy

ESKANDARI KHADIJEH, GHOURCHIAN HEDAYATOLLAH, BANAEI AGHDAS. ELECTROCHEMICAL BEHAVIOR OF ADULT AND FETAL HEMOGLOBIN AT GOLD-COATED MAGNETIC IRON OXIDE NANOPARTICLE. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS[Internet]. 2017;4(3):669-674. Available from: https://sid.ir/paper/352938/en

IEEE: Copy

KHADIJEH ESKANDARI, HEDAYATOLLAH GHOURCHIAN, and AGHDAS BANAEI, “ELECTROCHEMICAL BEHAVIOR OF ADULT AND FETAL HEMOGLOBIN AT GOLD-COATED MAGNETIC IRON OXIDE NANOPARTICLE,” JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, vol. 4, no. 3, pp. 669–674, 2017, [Online]. Available: https://sid.ir/paper/352938/en

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