DETECTION OF ANTIBODIES AGAINST HYDATID CYST
FLUID ANTIGENS USING IMMUNOBLOT TECHNIQUE

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JALOSIAN F.; DALIMI - ASI A.; ZAVARAN HOSSEINI A.; JALOSIAN F.

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Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2004 | Volume:12 | Issue:2 Page(s): 8-8

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Title

DETECTION OF ANTIBODIES AGAINST HYDATID CYST FLUID ANTIGENS USING IMMUNOBLOT TECHNIQUE

Author(s)

JALOSIAN F. | DALIMI - ASI A. | ZAVARAN HOSSEINI A. | JALOSIAN F. | Issue Writer Certificate

Keywords

HYDATIDOSISQ3

IMMUNOBLOTQ4

SEROLOGYQ3

Abstract

Introduction: IMMUNOBLOT is recognized as a sensitive and specific serological method for diagnosis of human HYDATIDOSIS. To analyze the reactivity of hydatid patients sera with the subunits of 2 major hydatid cyst fluid antigens, (5 and B ) IMMUNOBLOT method was used. Material & Methods:ln this regard, 100 sera ,of which 40 sera from patients with surgically diagnosed HYDATIDOSIS, 20 sera from patients with parasitic disease other than HYDATIDOSIS, 20 sera from patients other than parasitic disease, and 20 sera from healthy controls were examined. Results: For 3 subunits of Ag B (12, 16, 20 kD) the sensitivities were 92.5%,84.5% and 87.5% respectively and their specificity for all of the 3 subunits was 100%. For 2 subunits of Ag 5, (55, 65 kD)the sensitivity was 100% and the specificities were 100% and 90% respectively. Conclusion: The results indicated that the best subunit for HYDATIDOSIS diagnosis is the 55 kD subunit of Ag 5. Meanwhile, a lower reactivity (84.5% of all hydatid sera) was observed with 16 kD .subunits of Ag B.

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References

A SIMPLE METHOD FOR PREPARATION OF HYDATID CYST B-GROUP ANTIGENS

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APA: Copy

JALOSIAN, F., DALIMI - ASI, A., ZAVARAN HOSSEINI, A., & JALOSIAN, F.. (2004). DETECTION OF ANTIBODIES AGAINST HYDATID CYST FLUID ANTIGENS USING IMMUNOBLOT TECHNIQUE. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 12(2), 8-8. SID. https://sid.ir/paper/357207/en

Vancouver: Copy

JALOSIAN F., DALIMI - ASI A., ZAVARAN HOSSEINI A., JALOSIAN F.. DETECTION OF ANTIBODIES AGAINST HYDATID CYST FLUID ANTIGENS USING IMMUNOBLOT TECHNIQUE. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2004;12(2):8-8. Available from: https://sid.ir/paper/357207/en

IEEE: Copy

F. JALOSIAN, A. DALIMI - ASI, A. ZAVARAN HOSSEINI, and F. JALOSIAN, “DETECTION OF ANTIBODIES AGAINST HYDATID CYST FLUID ANTIGENS USING IMMUNOBLOT TECHNIQUE,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 12, no. 2, pp. 8–8, 2004, [Online]. Available: https://sid.ir/paper/357207/en

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