IMMUNOBLOT ANALYSIS OF SAFFRON POLLEN PROTEINS RECOGNIZED BY HUMAN IGE ANTIBODIES

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VARASTEH A.R.; KAGHAZIAN H.; TAYEBI D.; MOGHADAM M.; SANKIAN M.

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Title

IMMUNOBLOT ANALYSIS OF SAFFRON POLLEN PROTEINS RECOGNIZED BY HUMAN IGE ANTIBODIES

Author(s)

Keywords

ALLERGENQ2

HYPERSENSITIVITYQ4

CROCUS SATIVUSQ3

SAFFRONQ4

Abstract

Pollen is one of the major causes of allergy worldwide. Pollen ALLERGENs can cause an immediate HYPERSENSITIVITY (type I, IgE-mediated) response in susceptible individuals. HYPERSENSITIVITY to SAFFRON pollen is a serious problem, especially among the people who working with SAFFRON flowers. The aim of this study was to identify protein with IgE binding activity (ALLERGENic protein) in a SAFFRON pollen extract. Proteins of the SAFFRON pollen extract recognized by human JgE antibodies were investigated by SDSPAGE and Western blotting. Proteins with IgE binding activity were identified with sera from 23 patients who suffered from continues sneezing, rhino rhea, conjunctivitis, throat irritation, shortness of breath during exposure with SAFFRON flowers. A chemiluminescent immuno-detection method was used to study IgE binding activity of SAFFRON pollen proteins with patientss sera.Six IgE immunoreactive proteins ranging in molecular weight from 13.5 to 85 KD were identified. From among of these proteins, four with apparent molecular weight of 13.5- 14, 19.5-20, 42-43 and 85 KD could be identified with more than 35% of our allergic patients sera. While two proteins with IgE binding activity (58 and 67 KD) could be demonstrated for only some of our SAFFRON allergic patients. In conclusion, in this study we demonstrated the presence of several IgE binding proteins in the total extract of SAFFRON pollen.

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APA: Copy

VARASTEH, A.R., KAGHAZIAN, H., TAYEBI, D., MOGHADAM, M., & SANKIAN, M.. (2005). IMMUNOBLOT ANALYSIS OF SAFFRON POLLEN PROTEINS RECOGNIZED BY HUMAN IGE ANTIBODIES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 7(4 (24)), 12-16. SID. https://sid.ir/paper/357975/en

Vancouver: Copy

VARASTEH A.R., KAGHAZIAN H., TAYEBI D., MOGHADAM M., SANKIAN M.. IMMUNOBLOT ANALYSIS OF SAFFRON POLLEN PROTEINS RECOGNIZED BY HUMAN IGE ANTIBODIES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2005;7(4 (24)):12-16. Available from: https://sid.ir/paper/357975/en

IEEE: Copy

A.R. VARASTEH, H. KAGHAZIAN, D. TAYEBI, M. MOGHADAM, and M. SANKIAN, “IMMUNOBLOT ANALYSIS OF SAFFRON POLLEN PROTEINS RECOGNIZED BY HUMAN IGE ANTIBODIES,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 7, no. 4 (24), pp. 12–16, 2005, [Online]. Available: https://sid.ir/paper/357975/en

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