ANALYSIS OF IL-4 MRNA LEVEL BY RT-PCR AND ASSOCIATION WITH IGE PRODUCTION IN DIFFERENT RAT STRAINS

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TAVAKOL AFSHARI J.; HUTCHINSON I.V.

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Journal: Iranian Journal of Basic Medical Sciences Year:2004 | Volume:7 | Issue:2 (22) Page(s): 7-12

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Title

ANALYSIS OF IL-4 MRNA LEVEL BY RT-PCR AND ASSOCIATION WITH IGE PRODUCTION IN DIFFERENT RAT STRAINS

Author(s)

TAVAKOL AFSHARI J. | HUTCHINSON I.V. | Issue Writer Certificate

Keywords

INTERLEUKIN-4Q3

IGEQ3

RT-PCRQ3

CYTOKINESQ4

Abstract

Background INTERLEUKIN-4 (IL-4) is a key anti-inflammatory cytokine and plays an important role in the cytokine network and in the Th1/Th2 balance. It is generated by helper T cells, and possibly in the early adaptive immune response by mast cells and basophiles, and like all cytokine generally expressed at low levels with a short half-life of the mRNA. There are controversial evidences suggesting the role of IL-4 and IL-13 in the pathogenesis of allergy and asthma. The purpose of this research was to indicate the role of IL-4 gene expression in allergic and non-allergic rat models. We tested three different rat strains, "allergic" high 19B producing BN (Brown Norway) strain rats, "non-allergic" low IGE responder PVG strain rats and low IGE producing WISTAR strain rats, for IL-4 mRNA and IL-4 protein expression by a semi-quantitative RT-PCR and ELISA methods. The results showed that BN generated the highest amount of IL-4 and mRNA expression level. WISTAR strain and PVG strain showed lower amount of IL-4 expression in all experiments and can be looked at the lowest IL-4 mRNA producer. Our results demonstrated the hypothesis that higher expression of IL-4 mRNA may be "allergic" and high IGE producing. Although we can not exclude the polymorphism of the IL-4 gene in the non-coding region and the role of other cytokine like IL-13 and TGF-B in the cytokine network.

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APA: Copy

TAVAKOL AFSHARI, J., & HUTCHINSON, I.V.. (2004). ANALYSIS OF IL-4 MRNA LEVEL BY RT-PCR AND ASSOCIATION WITH IGE PRODUCTION IN DIFFERENT RAT STRAINS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 7(2 (22)), 7-12. SID. https://sid.ir/paper/357976/en

Vancouver: Copy

TAVAKOL AFSHARI J., HUTCHINSON I.V.. ANALYSIS OF IL-4 MRNA LEVEL BY RT-PCR AND ASSOCIATION WITH IGE PRODUCTION IN DIFFERENT RAT STRAINS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2004;7(2 (22)):7-12. Available from: https://sid.ir/paper/357976/en

IEEE: Copy

J. TAVAKOL AFSHARI, and I.V. HUTCHINSON, “ANALYSIS OF IL-4 MRNA LEVEL BY RT-PCR AND ASSOCIATION WITH IGE PRODUCTION IN DIFFERENT RAT STRAINS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 7, no. 2 (22), pp. 7–12, 2004, [Online]. Available: https://sid.ir/paper/357976/en

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