EFFECT OF VARIOUS CONCENTRATIONS OF INHIBITORS ON THE EXPRESSION OF CHEMOKINE IP-10/MOB-1

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HASANSHAHI RAVIZI GH.H.; HAJIZADEH M.R.; MIRZAEI MOHAMMAD REZA; POURSHANAZARI A.A.; REZVANI M.E.; VAZIRI R.; DICKSON A.J.

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2007 | Volume:15 | Issue:2 Page(s): 50-56

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Title

EFFECT OF VARIOUS CONCENTRATIONS OF INHIBITORS ON THE EXPRESSION OF CHEMOKINE IP-10/MOB-1

Author(s)

Keywords

CXC CHEMOKINEQ3

IP-10/MOB-1. HEPATOCYTEQ3

SB203580Q3

MG132Q3

KN62Q3

STAUROSPORINEQ3

Abstract

Introduction: Chemokines are low molecular weight proteins (8-17kDa) with the main role of immune cells recruitment to injured tissues, IP-10/Mob-1 is a CXC CHEMOKINE and different cell systems in response to external stimulation produce this chemokine. Various signaling pathways are used by cell and tissue systems to regulate production of proteins e.g. chemokines. Therefore we have investigated some of these pathways leading to production of IP-10/Mob-1 by primary cultured hepatocytes.Methods: In the present study, hepatocyts were isolated from male Sprague Dawley rats and cultured on Weymouth medium in presence and absence of different inhibitors such as SB203580, MG132, KN62 and STAUROSPORINE for indicated time points. Supernatant medium of culture was centrifuged and proteins ere isolated by SDS-PAGE and transferred to nitrocellulose membranes, then membranes were incubated with IP-10/Mob-1 antibody overnight and followed by incubation with secondary antibody and then IP-1 0/ Mob-l was detected by ECL.Results: We showed that the expression of IP-l O/Mob-l has been decreased in presence of STAUROSPORINE (10 and 50mM), SB203580 (50 mM) MG132 (20mM) and KN62 (10 and 20 mM) but did not change in absence or lower concentrations of these inhibitors.Conclusion: Hence, these in vitro data may aid to a better understanding of the pathways in chronic liver injuries and identify clinical studies that may aid in treatment or prevention of these conditions. Furthermore, there may be further potential to prevent changes to hepatocyte phenotype and allow isolation of hepatocytes with a greater physiological phenotype. Therefore it could be concluded that all of these pathways are used by hepatocytes regarding expression of IP-10/Mob-1.

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APA: Copy

HASANSHAHI RAVIZI, GH.H., HAJIZADEH, M.R., MIRZAEI, MOHAMMAD REZA, POURSHANAZARI, A.A., REZVANI, M.E., VAZIRI, R., & DICKSON, A.J.. (2007). EFFECT OF VARIOUS CONCENTRATIONS OF INHIBITORS ON THE EXPRESSION OF CHEMOKINE IP-10/MOB-1. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 15(2), 50-56. SID. https://sid.ir/paper/35867/en

Vancouver: Copy

HASANSHAHI RAVIZI GH.H., HAJIZADEH M.R., MIRZAEI MOHAMMAD REZA, POURSHANAZARI A.A., REZVANI M.E., VAZIRI R., DICKSON A.J.. EFFECT OF VARIOUS CONCENTRATIONS OF INHIBITORS ON THE EXPRESSION OF CHEMOKINE IP-10/MOB-1. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2007;15(2):50-56. Available from: https://sid.ir/paper/35867/en

IEEE: Copy

GH.H. HASANSHAHI RAVIZI, M.R. HAJIZADEH, MOHAMMAD REZA MIRZAEI, A.A. POURSHANAZARI, M.E. REZVANI, R. VAZIRI, and A.J. DICKSON, “EFFECT OF VARIOUS CONCENTRATIONS OF INHIBITORS ON THE EXPRESSION OF CHEMOKINE IP-10/MOB-1,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 15, no. 2, pp. 50–56, 2007, [Online]. Available: https://sid.ir/paper/35867/en

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