ISOLATION AND PHYSIOLOGICAL CHARACTERIZATION OF YEASTS AND SELECTION OF THE BEST STRAINS FOR ETHANOL PRODUCTION AS FUEL

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SEYED SIAMDOUST E.; MALEKZADEH F.; FALLAHIAN F.; AMIRMOZAFARI N.; RAZAVI M.R.

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Journal: JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY) Year:2011 | Volume:20 | Issue:78/1 (BIOLOGY ISSU) Page(s): 71-86

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Title

ISOLATION AND PHYSIOLOGICAL CHARACTERIZATION OF YEASTS AND SELECTION OF THE BEST STRAINS FOR ETHANOL PRODUCTION AS FUEL

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Keywords

YEASTQ3

ETHANOLQ3

HIGH OSMOTIC PRESSUREQ3

HIGH TEMPERATUREQ3

FERMENTATIONQ3

Abstract

Introduction: Given growing world population, more energy consumption, limitation of oil reservoirs as energy providing sources and increase of fuel price in future, implantation of alternative energy is necessary. ETHANOL, a safe and renewable fuel which is mostly produced by YEASTs through FERMENTATION of sugars worldwide, is a very suitable substitute. In order to improve ETHANOL production, the study of physiological YEASTs characteristics has been more considered in recent years due to their important role in FERMENTATION and ETHANOL production. Aim: In this research, attempt was to isolate YEASTs from their habitat such as various fruits and fermented food materials, physiological characterization to find the physiologically high potent Strains for using in industrial production of ETHANOL.Materials and Methods: Samples were taken from different kinds of whey, cucumber brines, yoghourt, raisins, palm, red and white grape and apple, plum, peach. Serial dilution method was used for YEAST isolation, followed by purification after morphological investigation. To physiological characterization, their resistance to various ETHANOL concentrations (1-13)%(v/v) in GPY medium, HIGH OSMOTIC PRESSURE environments, growth in different temperature, FERMENTATION of various sugars, hydrolysis and metabolize various carbon and nitrogen sources and adapting vitamin free mediums were used. Based on these, the best strains were selected and 28S rDNA Ribotyping was used for introducing them. Results: 40 YEASTs colonies were isolated and purified. 4 strains named MS1, MS2, MS3, MS5 were selected on the basis of their physiological characteristics. ETHANOL resistance of these strains were 12%, 11%, 8%, 6% (v/v) and maximum growth temperature were 42, 37, 40, 37(°C) respectively. All of them grew on HIGH OSMOTIC PRESSURE environments and except MS5, vitamin free mediums. All 4 strains were able to use various carbon and nitrogen sources and fermented glucose, fructose and mannose. Galactose, Sucrose and raffinose were fermented by MS2 and MS3, while maltose was fermented by MS2, trehalose by MS3, Cellobiose by MS5. Identification of MS1, MS2, MS3, MS5 by 28S rDNA Ribotyping revealed 81%, 100%, 99%, 88% similarity with known YEAST strains, respectively.Conclusion: 4 Selected strains showed considerable resistance to HIGH OSMOTIC PRESSURE and ETHANOL in tolerance tests. There is a direct proportional between high tolerance to ETHANOL and sugar with production of ETHANOL. So this can be concluded that these strains are useful for efficient ETHANOL production. Moreover, MS1 strain ability to grow at 42°C, fermenting of various sugars by MS2, MS3 strains and cellobiose by MS5 strain can be helpful for ETHANOL production. Identification of 4 strains revealed species genus of MS2 and MS3 strains but MS1 and MS5 strains are belonging to new species, genus even higher new taxonomical levels; In this case further investigations are needed.

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APA: Copy

SEYED SIAMDOUST, E., MALEKZADEH, F., FALLAHIAN, F., AMIRMOZAFARI, N., & RAZAVI, M.R.. (2011). ISOLATION AND PHYSIOLOGICAL CHARACTERIZATION OF YEASTS AND SELECTION OF THE BEST STRAINS FOR ETHANOL PRODUCTION AS FUEL. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 20(78/1 (BIOLOGY ISSU)), 71-86. SID. https://sid.ir/paper/359601/en

Vancouver: Copy

SEYED SIAMDOUST E., MALEKZADEH F., FALLAHIAN F., AMIRMOZAFARI N., RAZAVI M.R.. ISOLATION AND PHYSIOLOGICAL CHARACTERIZATION OF YEASTS AND SELECTION OF THE BEST STRAINS FOR ETHANOL PRODUCTION AS FUEL. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)[Internet]. 2011;20(78/1 (BIOLOGY ISSU)):71-86. Available from: https://sid.ir/paper/359601/en

IEEE: Copy

E. SEYED SIAMDOUST, F. MALEKZADEH, F. FALLAHIAN, N. AMIRMOZAFARI, and M.R. RAZAVI, “ISOLATION AND PHYSIOLOGICAL CHARACTERIZATION OF YEASTS AND SELECTION OF THE BEST STRAINS FOR ETHANOL PRODUCTION AS FUEL,” JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), vol. 20, no. 78/1 (BIOLOGY ISSU), pp. 71–86, 2011, [Online]. Available: https://sid.ir/paper/359601/en

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