Expression of miR-125 and MCL-1 and BCL-2 Anti-apoptotic Genes under the Influence of Valproic Acid Treatment in Breast Cancer Cell Lines

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Injinari Nastaran; Amini Farsani Zeinab; TEIMORI HOSSEIN

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2019 | Volume:37 | Issue:542 Page(s): 1061-1067

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Title

Expression of miR-125 and MCL-1 and BCL-2 Anti-apoptotic Genes under the Influence of Valproic Acid Treatment in Breast Cancer Cell Lines

Author(s)

Injinari Nastaran | Amini Farsani Zeinab | TEIMORI HOSSEIN | Issue Writer Certificate

Keywords

ApoptosisQ1

Breast cancerQ2

miRNAQ1

Valproic acidQ1

Abstract

Background: One of the causes of death through Breast cancer is drug resistance and the anti-apoptotic activity of cancer cells. Epigenetic factors are involved in both of these processes. Micro RNAs (miRNAs) are epigenetic agents that accelerate the inappropriate expression of cancer. Valproic acid is a histone deacetylase inhibitor that has anticancer activity. The aim of this study was to evaluate the effect of Valproic acid on miR-125b expression as one of the tumor suppressor miRNAs in Breast cancer, and the expression of BCL-2 and MCL-1 anti-apoptotic genes as possible targets of miR-125b in MDA-MB-231 and MCF-7 cell lines. Methods: In this experimental study, MDA-MB-231 and MCF-7 cell lines were first cultured in optimal conditions. Cell viability was measured at different concentrations of Valproic acid at 48 and 72 hours using MTT assay. The expression of miR-125b and BCL-2 and MCL-1 genes was analyzed using real-time polymerase chain reaction (PCR). The data were analyzed using one-way ANOVA test. Findings: Valproic acid significantly decreased the viability of both cell lines in dose-and time-dependent manner. It also led to a significant increase in miR-125b in both cell lines (P < 0. 010). In MCF-7 cells, this drug led to a significant decrease in BCL-2 and MCL-1 genes (P < 0. 001). But the difference in these two genes in MDA-MB-231 treated cells was not significant compared to the control group (P > 0. 050). Conclusion: Valproic acid may be suggested as a profitable option in cancer research, through its involvement in epigenetic processes, and its influence on the expression of BCL-2 and MCL-1 genes.

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APA: Copy

Injinari, Nastaran, Amini Farsani, Zeinab, & TEIMORI, HOSSEIN. (2019). Expression of miR-125 and MCL-1 and BCL-2 Anti-apoptotic Genes under the Influence of Valproic Acid Treatment in Breast Cancer Cell Lines. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 37(542 ), 1061-1067. SID. https://sid.ir/paper/362019/en

Vancouver: Copy

Injinari Nastaran, Amini Farsani Zeinab, TEIMORI HOSSEIN. Expression of miR-125 and MCL-1 and BCL-2 Anti-apoptotic Genes under the Influence of Valproic Acid Treatment in Breast Cancer Cell Lines. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2019;37(542 ):1061-1067. Available from: https://sid.ir/paper/362019/en

IEEE: Copy

Nastaran Injinari, Zeinab Amini Farsani, and HOSSEIN TEIMORI, “Expression of miR-125 and MCL-1 and BCL-2 Anti-apoptotic Genes under the Influence of Valproic Acid Treatment in Breast Cancer Cell Lines,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 37, no. 542 , pp. 1061–1067, 2019, [Online]. Available: https://sid.ir/paper/362019/en

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