Rh blood group genotyping in alloimmunized thalassemic patients in Tehran Adult Thalassemia Clinic during 2017-18

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Dadkhah Tehrani R.; OODI A.; AZARKEIVAN A.; AMIRIZADEH N.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2020 | Volume:17 | Issue:1 Page(s): 36-46

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Title

Rh blood group genotyping in alloimmunized thalassemic patients in Tehran Adult Thalassemia Clinic during 2017-18

Author(s)

Dadkhah Tehrani R. | OODI A. | AZARKEIVAN A. | AMIRIZADEH N. | Issue Writer Certificate

Keywords

ThalassemiaQ3

GenotypeQ2

Blood GroupsQ2

Abstract

Background and Objectives: The main antigens of the Rh blood group system (D, C, c, E, e) after the ABO system, are the most immune antigens. Due to existence of donor's red blood cells in the patient's circulation serologic methods can not accurately detect blood group antigens in patients with chronic blood transfusions, but molecular methods can overcome many of these limitations. Materials and Methods: In this cross sectional descriptive study, peripheral blood samples were taken from two hundred of alloimmunized thalassemic patients including 81 (40. 5%) male and 119 (59. 5%) female with mean age of 30 ± 10. 9 (age range 4-65) at Tehran Thalassemia Clinic during 1976-96. Phenotyping was done for C, c, E, e antigens. Sequence Specific Primers (SSPs)-PCR was performed and the discrepant results between the phenotype and genotyping were confirmed by DNA sequencing. Results: In this study, the highest prevalence of alloantibodies in the Rh system pertained to Anti-E 22% (44 patients) and Anti-D 10% (20 patients) and the frequency of the alleles in this blood group was determined C 71% (142), c 72. 5% (145), E 23% (46), e 98% (196). Thirty eight out of 200 patients had different results between serology and Genotype. Discrepancies were 7. 5% (14) (RHC), 6% (11) (RHc), 9. 5% (17) (RHE), and 1. 5% (3) (RHe). Conclusions: Molecular methods can help to determine the exact antigens as a complementary method by solving the problems of the serological method.

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APA: Copy

Dadkhah Tehrani, R., OODI, A., AZARKEIVAN, A., & AMIRIZADEH, N.. (2020). Rh blood group genotyping in alloimmunized thalassemic patients in Tehran Adult Thalassemia Clinic during 2017-18. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 17(1 ), 36-46. SID. https://sid.ir/paper/363122/en

Vancouver: Copy

Dadkhah Tehrani R., OODI A., AZARKEIVAN A., AMIRIZADEH N.. Rh blood group genotyping in alloimmunized thalassemic patients in Tehran Adult Thalassemia Clinic during 2017-18. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2020;17(1 ):36-46. Available from: https://sid.ir/paper/363122/en

IEEE: Copy

R. Dadkhah Tehrani, A. OODI, A. AZARKEIVAN, and N. AMIRIZADEH, “Rh blood group genotyping in alloimmunized thalassemic patients in Tehran Adult Thalassemia Clinic during 2017-18,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 17, no. 1 , pp. 36–46, 2020, [Online]. Available: https://sid.ir/paper/363122/en

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