MOLECULAR BLOOD GENOTYPING IN PATIENTS WITH THALASSEMIA MAJOR IN TEHRAN ADULT THALASSEMIC CLINIC

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHAIGAN MOZHGAN; SAMIEI SH.; AZAR KEYVAN A.; DANEILS J.; MARTIN P.; ATAEI ZAHRA; LOTFI P.; KAVARI M.; LOTFI H.; KASRAEIAN L.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2009 | Volume:6 | Issue:2 (23) Page(s): 107-115

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

2,425

Download:

Cites:

Information Journal Paper

Title

MOLECULAR BLOOD GENOTYPING IN PATIENTS WITH THALASSEMIA MAJOR IN TEHRAN ADULT THALASSEMIC CLINIC

Author(s)

Keywords

THALASSEMIAQ3

POLYMERASE CHAIN REACTIONQ3

BLOOD GROUP ANTIGENSQ3

Abstract

Background and Objectives: Blood typing by serologic methods after transfusion has limitations due to presence of donor red cells in recipients. Accurate determination of red blood cells (RBCs) antigens is very important in multitransfused patients including beta-thalassemics and sickle cell anemics. So, the aim of this study was to evaluate DNA- based methods as supplement to the hemagglutination technique to determine the red blood cell (RBC) antigen profile of multitransfused patients with beta- THALASSEMIA.Materials and Methods: DNA was extracted from peripheral blood of 20 apparently normal people and 44 patients including 35 with beta- THALASSEMIA (out of whom 19 had clinical evidence of delayed hemolytic transfusion reaction) , 8 with THALASSEMIA intermedia (out of whom 2 had hemolytic reaction), and one with sickle cell THALASSEMIA. RHD/ RHC/ RHc/ RHE/ RHe/ JKA/ JKB/ FYA/ FYB/ KELL1/ KELL2 alleles were determined by PCR and were then compared with the hemagglutination method.Results: Phenotype and genotype results were the same in all controls. The phenotypes and genotypes of 53 blood antigens of 26 patients were incompatible. Most of the discrepancies (19 cases) occurred in the Rh system, and fifteen in the Duffy and Kidd systems.Conclusions: The results show that screening platelet concentrates for bacterial contamination is necessary for blood transfusion centers and hospital blood banks. Blood typing by serologic method was not accurate in this study but genotyping could determine true blood groups in multitransfused patients and help in selection of RBCs without alloimmunized antigens in future transfusion attempts. Specificity, sensitivity, positive and negative predictive values of hemagglutination method for RhD antigen had good values in comparison to the molecular method. This might be due to pre- transfusion determination of RhD for thalassemic patients so as to receive Rh- matched blood units. It seems pre-transfusion blood typing of Rh and Kell antigens, which are the cause of hemolytic reactions, in comparison to the molecular method could be cost effective. In addition, typing of Rh and Kell antigens in some regular blood donors could be helpdul for selecting antigen-negative RBCs for transfusion dependent patients.

Cites

No record.

References

No record.

Cite

APA: Copy

SHAIGAN, MOZHGAN, SAMIEI, SH., AZAR KEYVAN, A., DANEILS, J., MARTIN, P., ATAEI, ZAHRA, LOTFI, P., KAVARI, M., LOTFI, H., & KASRAEIAN, L.. (2009). MOLECULAR BLOOD GENOTYPING IN PATIENTS WITH THALASSEMIA MAJOR IN TEHRAN ADULT THALASSEMIC CLINIC. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 6(2 (23)), 107-115. SID. https://sid.ir/paper/78349/en

Vancouver: Copy

SHAIGAN MOZHGAN, SAMIEI SH., AZAR KEYVAN A., DANEILS J., MARTIN P., ATAEI ZAHRA, LOTFI P., KAVARI M., LOTFI H., KASRAEIAN L.. MOLECULAR BLOOD GENOTYPING IN PATIENTS WITH THALASSEMIA MAJOR IN TEHRAN ADULT THALASSEMIC CLINIC. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2009;6(2 (23)):107-115. Available from: https://sid.ir/paper/78349/en

IEEE: Copy

MOZHGAN SHAIGAN, SH. SAMIEI, A. AZAR KEYVAN, J. DANEILS, P. MARTIN, ZAHRA ATAEI, P. LOTFI, M. KAVARI, H. LOTFI, and L. KASRAEIAN, “MOLECULAR BLOOD GENOTYPING IN PATIENTS WITH THALASSEMIA MAJOR IN TEHRAN ADULT THALASSEMIC CLINIC,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 6, no. 2 (23), pp. 107–115, 2009, [Online]. Available: https://sid.ir/paper/78349/en

Related Journal Papers