INVESTIGATING EFFECTS OF GELATIN-CHITOSAN FILM ON CULTURE OF BONE MARROW STROMAL CELLS IN RAT

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KARAMI JOYANI A.; SABERI M.; KAKA GH.; SADRAI SH.; JAFARI M.; SALEM F.; EMAMGHOLI A.

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Title

INVESTIGATING EFFECTS OF GELATIN-CHITOSAN FILM ON CULTURE OF BONE MARROW STROMAL CELLS IN RAT

Author(s)

Keywords

BONE MARROW STROMAL CELLQ3

GELATIN- CHITOSAN- FILMQ2

CELL PROLIFERATION AND DIFFERENTIATIONQ2

Abstract

Introduction: Gelatin and Chitosan are known as biodegradable and biocompatible biopolymers. These biopolymers have recently received increasingly more attention for tissue engineering. The aim of this study was to survey of effects of Gelatin-Chitosan film in viability, proliferation, apoptosis and differentiation on BONE MARROW STROMAL CELL (BMSCs) culture in rat.Methods: Fist, gelatin- chitosan composites film were prepared by solution mixing, of both biopolymer in %75 acetic acid. After two passage of BMSCs culture, cells in the four groups including control, gelatin, chitosan and chitosan-gelatin were grown. The cells viability during the second, fourth and sixth days by tripanblue, proliferation by cell account and cell apoptosis by Acridin Orange were examined. Also cell differentiation during the second, fourth and sixth day were evaluated by immunocytochemistry.Results: The results showed significant reduction in cell proliferation in chitosan alone group (P<0.05). But the gelatin and chitosan-gelatin groups were similar to the control group as the cell proliferation was increased. Also all groups had no cell differentiation.Conclusion: Results of proliferation, differentiation and apoptosis cultured BMSCs on a gelatin-chitosan film showed that gelatin-chitosan film can be used as a good model of a biodegradable scaffold in tissue engineering and cell therapy.

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APA: Copy

KARAMI JOYANI, A., SABERI, M., KAKA, GH., SADRAI, SH., JAFARI, M., SALEM, F., & EMAMGHOLI, A.. (2015). INVESTIGATING EFFECTS OF GELATIN-CHITOSAN FILM ON CULTURE OF BONE MARROW STROMAL CELLS IN RAT. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 22(6), 1673-1681. SID. https://sid.ir/paper/36319/en

Vancouver: Copy

KARAMI JOYANI A., SABERI M., KAKA GH., SADRAI SH., JAFARI M., SALEM F., EMAMGHOLI A.. INVESTIGATING EFFECTS OF GELATIN-CHITOSAN FILM ON CULTURE OF BONE MARROW STROMAL CELLS IN RAT. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2015;22(6):1673-1681. Available from: https://sid.ir/paper/36319/en

IEEE: Copy

A. KARAMI JOYANI, M. SABERI, GH. KAKA, SH. SADRAI, M. JAFARI, F. SALEM, and A. EMAMGHOLI, “INVESTIGATING EFFECTS OF GELATIN-CHITOSAN FILM ON CULTURE OF BONE MARROW STROMAL CELLS IN RAT,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 6, pp. 1673–1681, 2015, [Online]. Available: https://sid.ir/paper/36319/en

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