The Survey of Contamination with Genital Mycoplasma among Infertile Women with Bacterial Vaginosis by PCR

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Ahmadiyan fard Zohreh; Moazami Amin

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Title

The Survey of Contamination with Genital Mycoplasma among Infertile Women with Bacterial Vaginosis by PCR

Author(s)

Ahmadiyan fard Zohreh | Moazami Amin | Issue Writer Certificate

Keywords

Ureaplasma urealyticumQ2

Mycoplasma genitaliumQ1

Bacterial vaginosisQ3

PCRQ2

KermanQ3

Abstract

Introduction: Ureaplasma urealyticum and Mycoplasma genitalium are the most important opportunistic pathogens in the female reproductive system and the causative agent of the pelvic infection, non-gonococcal urethritis, abortion and infertility. The aim of this study was to the survey of contamination with genital mycoplasma among infertile women with bacterial vaginalis by PCR method in Kerman. Methods: In this cross-sectional study, the endo cervical swab sample from 60 women with vaginal infection (vaginitis and cervicitis) referred to the diagnostic laboratories of infertility treatment centers in Kerman in 2013 was prepared. The specimens were transferred to the laboratory in the transport medium and the DNA extracted from the specimens was analyzed as a template for amplification of the 16S rRNA encoding gene in the PCR reaction. Data were analyzed by SPSS V. 21 software. Results: Among 60 samples, 38% (23 sample) of the samples had mycoplasma contamination and Ureaplasma urealyticum and Mycoplasma genitalium bacteria were found in 8 sample (13. 32%) and 7 sample (11. 6%) of patients, respectively. Conclusion: Considering the potential effects of mycoplasmas on the complications of infection in maternal pregnancies and infant mortality, Therefore, the need for rapid diagnosis of this infection is felt more than ever. From the two microbial agents studied in this study, the possibility of infertility caused by infection with Ureaplasma urealyticum increased significantly, but more studies are needed.

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APA: Copy

Ahmadiyan fard, Zohreh, & Moazami, Amin. (2020). The Survey of Contamination with Genital Mycoplasma among Infertile Women with Bacterial Vaginosis by PCR. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 26(6 ), 789-795. SID. https://sid.ir/paper/363747/en

Vancouver: Copy

Ahmadiyan fard Zohreh, Moazami Amin. The Survey of Contamination with Genital Mycoplasma among Infertile Women with Bacterial Vaginosis by PCR. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;26(6 ):789-795. Available from: https://sid.ir/paper/363747/en

IEEE: Copy

Zohreh Ahmadiyan fard, and Amin Moazami, “The Survey of Contamination with Genital Mycoplasma among Infertile Women with Bacterial Vaginosis by PCR,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 6 , pp. 789–795, 2020, [Online]. Available: https://sid.ir/paper/363747/en

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